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园艺学报 ›› 2001, Vol. 28 ›› Issue (5): 415-420.

• 研究论文 • 上一篇    下一篇

玻里马胞质大白菜雄性不育系CMS3411-7温度敏感特性的研究

张鲁刚;郝东方;柯桂兰   

  1. ( 西北农林科技大学园艺学院, 杨凌712100)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-25 发布日期:2001-10-25

Characteristics of Sterility in CMS 3411-7 of Chinese Cabbage Sensitive to Low Temperature

Zhang Lugang, Hao Dongfang, and Ke Guilan
  

  1. ( College of Horticulture, Northwest Sci􀀁tech University of Agriculture & Forestry , Yangling 712100 )
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

摘要: 采用光照培养箱恒温、变温和田间自然变温, 研究大白菜玻里马胞质雄性不育系CMS 411-7 温度敏感的特性。结果表明: 在3~ 15内CMS 3411-7 均有育性转换, 其中昼/夜温度8/ 4~ 14/ 10 ( 即日平均温度6~ 12 ) 是有效转换的温度范围。在恒温6条件下经过3~ 5 d 处理时CMS 3411-7 的育性转换值( 不育级别) 可达到2 级, 该温度下随着处理时间延长( 3~ 9 d) 不育性转换程度逐渐加重。在9恒温下处理6 d 较处理3 d 和处理9 d 时的
转换程度都大。在14/ 10下转换程度与处理时间无关。在17/ 13 下处理时间延长反而抑制不育性转换。虽然不同处理下CMS 3411-7 不育性转换开始和转换结束的时间差异较大, 但显著转换发生的时间一般稳定在温度处理后10~ 27 d, 表现相对集中。就育性转换的启动来看,经过3 d 处理后8/ 4~ 17/ 13下均发生了育性转换, 说明3 d 处理就可以启动育性转换基因。

关键词: 大白菜, 雄性不育性, 不育系, 胞质雄性不育系, 育性转换, 温度敏感

Abstract: The effect of temperature and treating time on the expression of sterility of CMS 3411-7 in Chinese cabbage Petsai with Polima cytoplasm was observed with alternat ing temperature, constant temperature in light growth incubator and alternating temperature in nature condit ion. The results showed that range of temperature affecting the sterility of CMS 3411-7 was wide, it occurred sterility changeover or fluctuation in constant temperature 3- 15, the range of 8/ 4 to 14/ 10 ( day/ night, that is daily mean temperature 6- 12 ) is the effective temperature. At constant temperature
6 ! , the value of changeover of CMS 3411-7 got grade 2 in 3- 5 days treatment , and the changeover of it was enhanced while the treating time prolonged ( from 3 to 9 days) . At constant temperature 9, there was a largest changeover in 6 days treatment compared with that in 3 or 9 days treatment. At al ternating temperature 14/ 10, the intensity of changeover of sterility is not depended upon the treating time. At 17/ 13 long treating time inhibited the changeover of sterility. In addition, although there was large difference between the begin or the end of changeover of sterility in difference treatment, the time of significant changeover of sterility was still concentrated and stable in 10- 27 days after temperature treatment. Because the changeover of sterility happened in 8/ 4- 17/ 13 in only 3
days treatment , it seems that 3 days treatment in that condition is enough to induce the gene of changeover of sterility, whether the time shorter than 3 days may induce it still need future study.

Key words: Chinese cabbage [ Brassica campestris L. ssp. pekinensis ( Lour ) Olsson], Male sterility, Sterile line, Cytoplasmic Male Steridity, Changeover of sterility, Thermo􀀁, sensitive

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