抗菌肽MB39基因导入‘皇家嘎啦’苹果及其四倍体植株的培育

园艺学报 ›› 2001, Vol. 28 ›› Issue (5): 392-398.

抗菌肽MB₃₉基因导入‘皇家嘎啦’苹果及其四倍体植株的培育

刘庆忠¹;赵红军¹;刘　鹏¹;Mong Rengong²;Freddi A. Hammerschlag³

(¹山东省果树研究所, 泰安271000 ;　²USDA/ ARS HCRL , Corvallis , OR 97330 , USA;　3USDA/ ARS , Fruit Laboratory , Beltsville , MD 20705 , USA)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-25 发布日期:2001-10-25

Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple

Liu Qingzhong¹ , Zhao Hongjun¹ , Liu Peng¹ , Mong Rengong² , and Freddi A. Hammerschlag³

(¹ Shandong Institute of pomology , Tai’an , Shandong 271000;　² USDA/ ARS HCRL , Corvallis , OR 97330 , USA ;　³ USDA/ ARS , Fruit Laboratory , Beltsville , MD 20705 , USA)

Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

摘要/Abstract

摘要： 采用农杆菌介导法, 将抗菌肽MB₃₉基因转入‘皇家嘎啦’苹果, 获得了7 个转基因株系。通过秋水仙素诱变染色体组工程育种技术, 由转化体叶片获得了20 个四倍体植株。经PCR 检测和Southern Blotting 杂交证明, 抗菌肽MB₃₉基因已经整合到皇家嘎啦苹果的染色体组中。转基因株系TR21、TR23 提高了对火疫病的抗性。采用流式细胞技术( Flow Cytometry) 确定植株为四倍体。

关键词: 抗菌肽MB39, 转基因, 苹果, 四倍体, 秋水仙素

Abstract: As part of the program to develop transgenic Malus ×Domestic. cv. ‘Royal Gala’tetraploid with improved traits , new germplasmof transgenic diploid and tetraploid plants with cecropin MB39 gene were regenerated. Seven transgenic diploid plants were obtained from etiolated internodal
explants by Agrobacterium tumefaciens-mediated transformation using the plasmid binary vector pGV containing a chimeric gene cecropin MB39 . The integration of the cecropin gene into apple genome was confirmed by PCR and Southern blotting analysis. It was proved that lines , TR-1 and TR-3 , had increased the resistance to fireblight disease caused by Erwinia amylovora. Twenty tetraploid lines were produced by cocultivating leaf explants from wild type and transgenic diploid shoots with colchicine in apple regeneration medium. Flow cytometry was used for ploidy etermination. The tetraploid plants were distinguishable from the diploid on morphological as well as cytogenetic grounds. Both the transgenic diploid and tetraploid plants are now being evaluated for growth development and disease resistance.

Key words: Cecropin MB₃₉, Transgenic, Apple, Tetraploid, Colchicine

中图分类号:

刘庆忠;赵红军;刘　鹏;Mong Rengong;Freddi A. Hammerschlag. 抗菌肽MB₃₉基因导入‘皇家嘎啦’苹果及其四倍体植株的培育[J]. 园艺学报, 2001, 28(5): 392-398.

Liu Qingzhong;Zhao Hongjun;Liu Peng;Mong Rengong;and Freddi A. Hammerschlag. Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple[J]. ACTA HORTICULTURAE SINICA, 2001, 28(5): 392-398.

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抗菌肽MB₃₉基因导入‘皇家嘎啦’苹果及其四倍体植株的培育

Regeneration of Tetraploid Plants with Cecropin MB₃₉ Gene from‘Royal Gala’Apple

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