https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2001, Vol. 28 ›› Issue (4): 342-344.

• 研究报告 • 上一篇    下一篇

桃离体组织分化再生植株的研究

张永庆1;陈大明1;金勇丰2 ;张上隆1 , *   

  1. (1 浙江大学园艺系, 杭州310029 ; 2 浙江大学生物化学研究所, 杭州310029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-08-25 发布日期:2001-08-25

Regeneration of Peach Plantlet from Callus Derived from Explant

Zhang Yongqing1 , Chen Daming1 , Jin Yongfeng2 , and Zhang Shanglong1
  

  1. (1 Department of Horticulture , Zhejiang University , Hangzhou 310029; 2 Institute of Biochemistry , Zhejiang University , Hangzhou 310029)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

摘要: 以奉化‘玉露’桃叶片、幼茎、下胚轴、子房、胚珠、胚乳及花后45~50 d 和75 d 的未成熟胚、完全成熟的种胚为外植体, 进行离体培养再分化试验。结果表明只有花后45~50 d 和75 d 的未成熟胚产生白色致密节球状的愈伤组织, 并可诱导出不定芽, 不定芽分化率分别为73. 8 %和15. 5 %; 62BA 诱导愈伤组织不定芽的效果优于KT。不定梢转移生根成苗获得了完整的再生植株。探讨了不同激素和碳源等对不定芽诱导的影响。

关键词: 桃, 外植体, 愈伤组织, 再分化, 再生植株, 组织培养

Abstract: Various explants of ‘Yulu’peach including leave , young shoot , hypocotyl , ovule , ovary , endosperm, immature embryo collected approximately 45 - 50 days or 75 days after full bloom , and mature embryo were cultured in vitro. As a result , only the white nodular callus derived from immature embryos collected 45 - 50 days and 75 days after bloom succeeded in producing adventitious buds , and the regeneration rates of white callus were 73. 8 %
and 15. 5 % respectively. 62BA had stronger effect than KT in inducing adventitious bud formation. Adventitious shoots were transferred and cultured to plantlets. Effect of different hormone , carbon source , medium and transfer time on redifferentiation of adventitious buds from callus derived from immature embryos have been discussed.

Key words: Peach ( Prunus persica), Explant, Callus, Redifferentiation, Plantlet, Tissue culture

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