食用仙人掌的离体培养及其快速繁殖

园艺学报 ›› 2001, Vol. 28 ›› Issue (4): 327-330.

食用仙人掌的离体培养及其快速繁殖

陈丽静¹;潘　英²;马　慧¹;刘少霞¹;姜明兰¹;钟文田¹

(¹ 沈阳农业大学生物科学技术学院, 沈阳110161 ;　² 沈阳农业大学生物科学技术学院海城天成产学研基地, 海城114200)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-08-25 发布日期:2001-08-25

Tissue Culture and High Frequency Propagation of Opuntia milpa altaHaw.

Chen Lijing , Pan Ying , Ma Hui , Liu Shaoxia , Jiang Minglan , and Zhong Wentian

( Biotechnology Institute of Shenyang Agricultural University , Shenyang 110161)

Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

摘要/Abstract

摘要： 研究了食用仙人掌离体快繁的若干影响因素。适宜的培养基为: 不定芽诱导, MS + 6-BA 3. 0 mg/L + NAA 0. 1 mg/L ; 继代和增殖, MS + 6-BA 1 mg/L + KT 0. 5 mg/L + NAA
0. 3 mg/L , 继代培养30 d , 平均增殖倍数为8 ; 生根培养基为1/ 2 MS + NAA 0. 1 mg/L + IBA 0. 1 mg/L。在培养过程中, 有些外植体膨大, 并有疏松的淡黄色颗粒状愈伤组织出现, 从愈伤组织表面分化出芽; 有些外植体不膨大, 无愈伤组织出现, 直接分化出丛生芽。过渡移栽基质为田园土∶珍珠岩∶河沙= 1∶1∶1 , 试管生根苗根长约1 cm时移栽, 成活率最高可达95 %以上。

关键词: 仙人掌, 食用仙人掌, 植物组织培养, 快速繁殖

Abstract: Effects of several factors such as medium , cutting materials and plant growth regulators on in vitro culture and rapid propagation of Opuntia milpa alta Haw. were investigated. Using the Opuntia milpa alta Haw. stem as explant , a method for high frequency propagation of Opuntia milpa alta Haw. was established. The results of the experiments suggested that the appropriate medium for different culture stages were : (1) Germination of adventitious buds : MS + 6-BA 3. 0 mg/ L + NAA 0. 1 mg/ L. On this medium shoot multiplication occurred at a 82fold rate after 30 days ; (2)
Differentiation and subculture of rosette buds : MS + 6-BA 1 mg/ L + KT 0. 5 mg/ L + NAA 0. 3 mg/L ; (3) Rootage : 1/ 2 MS + NAA 0. 1 mg/ L + IBA 0. 1 mg/ L. The shoots could be directly rooted in transplantation medium (soil , vermiculite and sand in equal proportion) with up to 95 % survival .

Key words: Opuntia Mill, Opuntia milpa alta Haw., Plant tissue culture, High frequencypropagation

中图分类号:

S 682

陈丽静;潘　英;马　慧;刘少霞;姜明兰;钟文田. 食用仙人掌的离体培养及其快速繁殖[J]. 园艺学报, 2001, 28(4): 327-330.

Chen Lijing;Pan Ying;Ma Hui;Liu Shaoxia;Jiang Minglan;and Zhong Wentian. Tissue Culture and High Frequency Propagation of Opuntia milpa altaHaw.[J]. ACTA HORTICULTURAE SINICA, 2001, 28(4): 327-330.

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