新高系梨10个品种S基因型的鉴定

园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1353-1360.

新高系梨10个品种S基因型的鉴定

袁德义¹;谭晓风^1*;张琳¹;赵思东¹;乌云塔娜¹;段经华¹;曹玉芬²

(¹中南林业科技大学经济林育种与栽培国家林业局重点实验室，长沙 410004；²中国农业科学院果树研究所，兴城 125100)

收稿日期:2007-05-07 修回日期:2007-09-30 出版日期:2007-12-25 发布日期:2007-12-25
通讯作者: 谭晓风

Identification of S-genotype of Ten Cultivars from Niitaka Line Pear

YUAN De-yi¹,TAN Xiao-feng^1*,ZHANG Lin¹,ZHAO Si-dong¹,WUYUN Ta-na¹, DUAN Jing-hua¹,and CAO Yu-fen²

(¹The Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-South of Forestry and Technology University, Changsha 410004, China;² Fruit Tree Research Institute，Chinese Academy of Agricultural Sciences，Xingcheng 125100，China)

Received:2007-05-07 Revised:2007-09-30 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 以亲缘关系极近的新高系梨10个品种及被视为‘新高’亲本的‘天之川’和‘今村秋’梨等为试验材料，对其基因组DNA进行了PCR-RFLP检测、DNA序列测定及生物信息学分析。结果表明：新高系梨10个品种即‘新高’、‘黄金’、‘水晶’、‘早生黄金’、‘早蜜新高’、‘天皇’、‘金秋’、‘晚大新高’、‘农家新高’和‘鲜黄’的S基因型分别为S₃S₉、S₃S₄、S₃S₉、S₃S₄、S₃S₉、S₃S₉、S₃S₉、S₃S₉、S₃S₉和S₃S₅；‘天之川’、‘今村秋’的S基因型分别为S₁S₉和S₁S₆，通过基因型判定‘今村秋’非‘新高’梨的父本。

关键词: 梨, 砂梨, 新高系梨, 自交不亲和性, S基因型, 鉴定

Abstract: Ten cultivars with extremely close relationship from Niitaka line pear were used as materials for S-genotyping. In addition, two cultivars 'Imamuraaki' and 'Amanogawa' that were considered to be the parents of cultivar 'Niitaka' were also included. PCR-RFLP analysis of genomic DNAs of the 12 cultivars, nucleotide sequencing of amplified fragments and sequence analysis were performed. As the results, the ten cultivars of Niitaka line were genotyped as follows: 'Niitaka' (S₃S₉), 'WhangKeumBae' (S₃S₄), 'Sujeong' (S₃S₉), 'Josengwhangkeum'(S₄S₉), 'Soumituniitaka'(S₃S₉), 'Yewang'(S₃S₉), 'Jinqiu'(S₃S₉), 'Banndainiitaka' (S₃S₉), 'Nokaniitaka'(S₃S₉), and 'Sunhwang' (S₃S₅). The S-genotype of 'Amanogawa' and 'Imamuraaki'was identified as S₁S₉and S₁S₆, respectively, suggesting that 'Imamuraaki'is unlikely to be the pollen parent of 'Niitaka'.

Key words: Pear, Pyrus pyrifolia, Niitaka line pear, Self-incompatibility, S-genotype, Identification

中图分类号:

S 661.2

袁德义;谭晓风;张琳;赵思东;乌云塔娜;段经华;曹玉芬. 新高系梨10个品种S基因型的鉴定[J]. 园艺学报, 2007, 34(6): 1353-1360.

YUAN De-yi;TAN Xiao-feng;ZHANG Lin;ZHAO Si-dong;WUYUN Ta-na;DUAN Jing-hua;and CAO Yu-fen. Identification of S-genotype of Ten Cultivars from Niitaka Line Pear[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1353-1360.

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