关键词: 辣椒, 高赖氨酸蛋白基因, RACE, 组织表达特征

Abstract: To obtain a new lysine-rich protein gene from species of solanaceae, a 390-bp fragment was amplified from pepper cultivar‘Jiangshu 7’using its matural pollen cDNA as the template and the conservative sequences of potato and tomato lysine-rich protein genes as the primers by RT-PCR. A full-length cDNA with completed open reading frame of 223 amino acids was cloned using the strategy of RACE(rapid amplification of cDNA ends ).This cDNA was designated as Cflr (GenBank accession number : EU367999 ), which contains 920 bp with an un-translated region of 84 bp at its 5＇end and a polyA tail at the 3＇end. BLAST search against NCBI showed that the Cflr gene shared 50%-60% identity with the lysine-rich protein genes from potato and tomato in nucleotide and 40%-50% in amino acid. The lysine content of CFLR protein was 21.2%，which was higher than the reported natural lysine-rich proteins, and the threonine content was 10.3%. Analysis of semi-quantitative RT-PCR indicated that Cflr gene was transcribed in matural pollen and petal largely, less in leave, and hardly in immature anther, stem and root.

Key words: pepper, lysine-rich protein gene, RACE, tissue expression characterization