https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2001, Vol. 28 ›› Issue (1): 59-61.

• 研究报告 • 上一篇    下一篇

沙田柚自交花柱中识别蛋白的免疫金定位

薛妙男;李义平;张杏辉;杨继华   

  1. (广西师范大学生物系, 桂林541004)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-02-25 发布日期:2001-02-25

Immuno-gold Localization of Recognition Prorein in the Self-Pollination Style of Shatinyu

Xue Miaonan, Li Yiping, Zhang Xinhui, and Yang Jihua   

  1. (Department of Biology , Guangxi Normal University , Guilin 541004)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

摘要: 应用免疫金标记抗体技术检测了沙田柚自花授粉后1~ 3 d 花柱1/ 2 处通道细胞中识别蛋白定位分布, 表明金颗粒依次定位于内质网, 高尔基体、纤维壁, 证实沙田柚花柱1/ 2 处通道细胞中金颗粒分布的变化与花粉管在花柱中生长受阻位点和时间一致。1/ 2 花柱通道细胞纤维壁上的糖蛋白是沙田柚不亲和反应的识别位点。

关键词: 柑桔类, 沙田柚, 自交不亲和, 花柱, 识别蛋白, 免疫金标记􀀁

Abstract: By immuno-gold, the recognition protein in the stylar passage cell of shatinyu (Citrus grandis var. Shatinyu Hort. ) 1- 3 days after self􀀁pollination was examined. The gold􀀁grains were located on the endoplasm reticu􀀁
lum, golgiosome and cellulose wall successively. The change of the gold􀀁grains distribution in the 1/ 2 stylar passage cell of shatinyu was identical to that of pollen tube stopping glycoprotein on the cellulose wall of 1/ 2 stylar passage cell which was the recognition site of self-incompatibility of shatinyu.

Key words: Citrus, Shatinyu (Citrus grandis var. Shatinyu Hort. ), Self-incompatibility, Style, Recognition protem, Immuno-gold

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