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园艺学报 ›› 2008, Vol. 35 ›› Issue (8): 1221-1226.

• 研究简报 • 上一篇    下一篇

万寿菊杂交一代遗传多态性的SRAP标记分析

张西西1,2;徐 进1;王 涛2;董爱香2;赵梁军1*   

  1. 1中国农业大学观赏园艺与园林系,北京100094;2北京市园林科学研究所,北京100102)
  • 收稿日期:2008-03-13 修回日期:2008-06-11 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 赵梁军

Genetic Diversity with Molecular Marker SRAP of Tagetes erecta L.

ZANG Xi-xi1,2,XU Xin1,WANG Tao2,DONG Ai-xiang2, and ZHAO Liang-jun1*
  

  1. (1Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094,China; 2Beijing Institute of Horticulture and Gardening,Beijing 100102,China)
  • Received:2008-03-13 Revised:2008-06-11 Online:2008-08-25 Published:2008-08-25
  • Contact: ZHAO Liang-jun

摘要:

应用SRAP (Sequence-Related Amplified Polymorphism) 分子标记对当前市场上推广的48个万寿菊杂交一代品种进行遗传多态性研究,用60个引物组合进行扩增,从中筛选到20个多态性引物组合,共产生289个多态性条带,平均每个引物组合产生14.45个多态性条带,显示了较高的多态性比率。聚类分析20个引物组合的扩增结果,48份材料分为两大类,Jaccard's相似系数在0.25~0.91之间。

关键词: 万寿菊, 杂交种, 多态性, SRAP

Abstract: Study on genetic diversity of Marigold could benefit the research of cultivar property right protecting,seanged purity testing,and genetic breeding. A new molecular marker system-SRAP (Sequence-Related Amplified Polymorphism) was firstly applied on 48 marigold hybrids genetic diversity research. 20 primer pairs selected from 60 amplified 289 polymorphic bands with an average of 14.45 polymorphic bands perprimer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 20 primer pairs showed 48 hybrids could be distinguished into two main groups. Jaccard s similarity coefficient ranged from 0.25-0.91.

Key words: Tagetes erecta L., Hybrid, Polymorphism, SRAP

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