关键词: 香蕉, 薄片培养, 器官发生

Abstract: The organogenesis of ‘Guangdong 631’banana by micro-cross sections culture and the factors influencing plant regeneration were investigated. The optimal culture condition was established by a statistical method and making research on the single factor : (1) the medium for direct organogenesis contained Ma (MS modified) , BAP 10μmol/ L , KT 50μmol/ L and IAA 1μmol/ L ;
(2) the medium for callus induction contained Mb (B₅ modified) , dicamba 10μmol/ L , IAA 1μmol/L , 2 , 4-D 0. 7μmol/ L , NAA 0. 5μmol/ L , BAP 4. 4μmol/ L and 1 g/ L activated charcoal ; (3) the medium for callus subculture contained Mb , 5μmol/ L dicamba , IAA 1μmol/ L , 2 ,4-D 0. 4μmol/ L , BAP 22μmol/ L , KNO₃ 500 mg/ L , vitamine 40 mg/ L B₁ and activated 0. 5μg/L charcoal ; (4) the medium for induction shoot buds from the callus was composed of halfstrength Ma , BAP 1μmol/ L , 0. 5μmol/ L IAA and 1 g/ L activated charcoal ; (5) the plant regeneration medium was composed of Ma , BAP 1μmol/ L , KT 4. 6μmol/ L and NAA 1μmol/ L. The callus induction and subculture was kept in darkness. The application of micro-cross sections of
banana for regeneration of plants might be a useful alternative to cell suspensions and protoplast culture in banana transformation.

Key words: Banana, Micro-cross sections culture, Organogenesis