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园艺学报 ›› 2005, Vol. 32 ›› Issue (3): 496-499.

• 研究报告 • 上一篇    下一篇

枣胚培技术体系的建立

杜学梅1;李登科1;王永康1;隋串玲1;樊保国2   

  1. (1 山西省农业科学院果树研究所, 太谷 030815; 2 山西师范大学, 临汾 041000)
  • 收稿日期:2004-08-12 修回日期:2005-01-11 出版日期:2005-06-25 发布日期:2005-06-25

Estabilishnent of Embryo Culture Technical System of Chinese Jujube ( Zizyphus jujuba Mill. )

Du Xuemei1;Li Dengke1;Wang Yongkang1;Sui Chuanling1;Fan Baoguo2   

  1. (1 Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu 030815, China; 2 Shanxi Normal Unirersity, Linfen 041000, China)
  • Received:2004-08-12 Revised:2005-01-11 Online:2005-06-25 Published:2005-06-25

摘要: 以‘六月鲜’枣为试材, 对花后12~82 d的幼胚进行培养。结果表明, 适宜于胚挽救的最小胚龄为花后50 d左右; 在胚形成前进行离体培养, 蔗糖浓度以7%为宜, 而胚形成后离体培养以5%蔗糖为宜; 暗培养可促进离体胚的生长发育, 且有利于生根; 植物生长调节剂及有机添加物极显著地影响着胚培效果。花后70~72 d胚龄的胚适宜培养基为1 /2MS + IBA 0.4 (mg/L, 单位下同) +BA 0.8 +LH 500 + GA35 + 5%蔗糖。胚培苗经扩繁后转入1 /2 MS + IBA 1.0 + IAA 0.06 + 2%蔗糖培养基上, 获得了健壮生根苗,生根苗移栽成活率达85%以上。

关键词: 枣, 胚, 组织培养

Abstract: The factors affecting embryo culture were studied in Zizyphus jujuba ‘Liuyuexian’. The youngest embryo saved successfullywas 50 days old. For the forming embryos, the op timal concentration of sucrose was 7% , for the formed ones was 5%. Dark treatment is beneficial to embryo sprouting, growing and rooting. There are extremely significance effects of plant growth regulators and organic substance on embryo culture. The media of 1 /2MS + IBA 0.4 (mg/L, the same below ) +BA 0.8 +LH 500 + GA3 5 + 5%
sucrose is suitable for culture of 70 - 72 days old embryo. The rootless seedling should be transferred to 1 /2 MS + IBA 1.0 + IAA 0.06 + 2% sucrose to induce normal root system.

Key words: Chinese jujube, Embryo, Tissue culture

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