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园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 486-487.

• 研究报告 • 上一篇    下一篇

莴苣‘红帆’品种下胚轴愈伤组织诱导与植株再生

高 辉;苟晓松;邓运涛;李旭锋*   

  1. (四川大学生命科学学院, 成都610064)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

In Vitro Plant Regeneration from Callus Induced from Hypocotyl of Lettuce(Lactuca sativa)

Gao Hui, Gou Xiaosong, Deng Yuntao, and Li Xufeng   

  1. (College of Life Science, Sichuan University , Chengdu 610064, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要: 莴苣下胚轴愈伤组织诱导时, MS+ 6-BA 0. 5 mg/ L+ 2, 4-D 1 mg/ L+ NAA 0. 1 mg/ L 效果最好,出愈率达94%; 愈伤组织分化为芽时, MS+ 6-BA 1. 0 mg / L+ NAA 0. 05 mg/ L 分化频率最高, 达100% ; 再生苗在1/ 2MS+ NAA 0. 5 mg/ L 中诱导生根成为完整植株。

关键词: 莴苣, 下胚轴, 愈伤组织, 植株再生

Abstract: Taking MS、1/ 2MS as basic media, adopting 6-BA、NAA、and 2, 4-D of different concentration composition to conduct t issue culture and plant regeneration from hypocotyl of lettuce, the result showed that medium of MS+ 6-BA 0. 5 mg/L+ 2, 4-D 1mg/L+ NAA 0. 1mg/ L , was the best to induce callus from hypocotyl, the rate of callus is 94%; and the differentiat ion medium, MS+ 6-BA 1. 0mg/ L + NAA 0. 05mg/ L, was the best to induce buds from callus and had a 100% plant regenerat ion rate; and it was for rooting media, 1/ 2MS+ NAA 0. 5 mg/ L, to induce root ing from seedlings.

Key words: Lettuce (Lactuca sativa), Hypocotyl, Callus tissue, Plant regeneration

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