Cucumis属植物种间正反杂交差异的研究

园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 483-485.

Cucumis属植物种间正反杂交差异的研究

陈劲枫;庄飞云;娄群峰;徐玉波;钱春桃;任　刚;罗向东

(作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院蔬菜系, 南京210095)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

Studies on Reciprocal Differences in Interspecific Hybridization in Cucumis

Chen Jinfeng, Zhuang Feiyun, Lou Qunfeng, Xu Yubo, Qian Chuntao, Ren Gang, and Luo Xiangdong

(State Key Lab of Crop Genetics and Germplasm Enhancement , Vegetable Research Institute , Nanjing Agricultural University , Nanjing210095 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要/Abstract

摘要： 在形态和分子两个水平上对栽培黄瓜(Cucumis sativus L. , 2n = 14) 与酸黄瓜(C. hystrixChakr. , 2n = 24) 正反杂交而成的双单倍体(2n = 19) 、双二倍体(2n = 38) 植株的形态和育性差异进行了比较研究。结果表明: 在形态上植株的分枝数、第1 朵雌花节位偏向于父性遗传, 主蔓节间长则偏向于母性遗传, 另外一些性状如主蔓直径呈中间型。通过RAPD 标记分析表明, 在所选的21 条随机引物中, 15条引物(占71 %) 显示出正反交之间的差异; 其中一些条带表现出父性遗传现象。在育性上, 正反交植株的表现截然不同。当以黄瓜为母本时, 杂种植株呈现雌雄高度不育; 而以酸黄瓜为母本时, 雌雄育性在染色体加倍后均得到恢复。

关键词: Cucumis属, 种间杂交, 正反交, RAPD

Abstract: A set of reciprocal crosses with different chromosome level made between Cucumis sativus and C.hystrix as the experiment system was used to investigate the difference of morphology , fertility and DNA. The
diameter of stem , the length of petiole , the shape and size of leaves were intermediated when compared to parents. The branching number and the appearance of first female flower showed paternal transmission while the length of inter-node of main stem showed maternal transmission in all of hybrids. The RAPD results indicated fifteen arbitrary primer band patterns were different among the four types of hybrids , and only six were the same. Some fragments obviously showed the paternal expression in the reciprocal cross. The fertility of reciprocal plants were great different . When C. hystrix used as female , the tetraploid (2n = 38) / diploid (2n = 19) plants had the higher fruit sets , and even the amphidiploidy plants could produce viable seeds. However , when the cucumber used as female , both tetraploid/ diploid plants were highly sterile and had very low fruit sets.

Key words: Cucumis, Interspecific hybridization, Reciprocal differences, RAPD

中图分类号:

S 642

陈劲枫;庄飞云;娄群峰;徐玉波;钱春桃;任　刚;罗向东. Cucumis属植物种间正反杂交差异的研究[J]. 园艺学报, 2002, 29(5): 483-485.

Chen Jinfeng;Zhuang Feiyun;Lou Qunfeng;Xu Yubo;Qian Chuntao;Ren Gang;and Luo Xiangdong. Studies on Reciprocal Differences in Interspecific Hybridization in Cucumis[J]. ACTA HORTICULTURAE SINICA, 2002, 29(5): 483-485.

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