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园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 732-734.

• 研究报告 • 上一篇    下一篇

蝴蝶兰组织培养中的褐化控制研究

刘真华1,2;葛 红1;郭绍霞2;刘洪涛1;曹灿景2;周玉杰1;李秋香1   

  1. 1 中国农业科学院蔬菜花卉研究所, 北京100081; 2 莱阳农学院环境艺术系, 莱阳265200
  • 收稿日期:2004-12-13 修回日期:2005-04-13 出版日期:2005-08-25 发布日期:2005-08-25

Studies of Antibrowning in the Tissue Culture of Phalaenopsis

Liu Zhenhua1, 2;Ge Hong1;Guo Shaoxia2;Liu Hongtao1;Cao Canjing2;Zhou Yujie1;Li Qiuxiang1   

  1. 1 Institute of Vegetables and Flowers, Chinese Academ y of Agricultural Sciences, Beijing 100081, China; 2 The Landscape Architecture and Art Department, Laiyang Agricultural College, Laiyang 265200, China)
  • Received:2004-12-13 Revised:2005-04-13 Online:2005-08-25 Published:2005-08-25

摘要: 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。

关键词: 蝴蝶兰, 组织培养, 褐化, 褐化控制

Abstract: In order to control the browning of tissue culture on Phalaenopsis, the contrast test of adsorbent: activated charcoal, PVP, and five antioxidants: citric acid, cysteine, glutathione, vitamin C, sodium thiosulfate in tissue culture of Phalaenopsis were studied. The results showed that activated charcoal can control browning and promote growth. But at the same time, it was adverse to the differentiation. Although the glutathione, which the effect of antibrowning was not as good as activated charcoal's, its resultant effect was the best one.

Key words: Phalaenopsis spp., Tissue culture, Browning, Antibrowning

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