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园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 701-703.

• 研究报告 • 上一篇    下一篇

大白菜高频再生体系的建立及策略

王 洋;崔继哲;李翠玲   

  1. (哈尔滨师范大学生物化学与分子生物学实验室, 哈尔滨150080)
  • 收稿日期:2005-01-17 修回日期:2005-06-07 出版日期:2005-08-25 发布日期:2005-08-25

Strategies in Establishing Effective Regeneration System of Chinese Cabbage

Wang Yang;Cui Jizhe;Li Cuiling   

  1. (Biochemistry and Molecular Biology Laboratory, Harbin Normal University, Harbin 150080, China)
  • Received:2005-01-17 Revised:2005-06-07 Online:2005-08-25 Published:2005-08-25

摘要: 就北方大白菜14个基因型进行组织培养再生比较, 以筛选出的较高再生率的基因型为试材,在含不同配比的6-BA、TDZ、NAA及AgNO3、GA3、ABA的培养基中进行再生比较, 分别建立了含有6-BA和TDZ的大白菜组织培养再生最佳培养基: MS + 6-BA 5 mg/L +NAA 0.5 mg/L +AgNO3 2 mg/L和MS + TDZ0.1 mg/L + 6-BA 5 mg/L +NAA 0.5 mg/L +AgNO3 2 mg/L +ABA 0.25 mg/L, 大白菜再生频率最高可以达到90.6% , 建立了大白菜高频再生体系, 讨论分析了影响大白菜再生的主导因素并提出建立大白菜高频再生体系的可行策略。

关键词: 大白菜, 基因型, 6-BA, TDZ, ABA

Abstract: This study evaluated shoot organogenic potential of 14 genotypes of Chinese cabbage (Brassica campestris L. ssp. pekinensis) , the regeneration frequency varied significantly. The genotype which had maximum shoot regeneration was chosen for investigating the op timum concentration of 6-BA or TDZ on shoot
regeneration. The maximum frequency of shoot regeneration was obtained respectively in the mediums: MS + 6-BA 5 mg/L +NAA 0.5 mg/L +AgNO3 2 mg/L and MS + TDZ 0.1 mg/L + 6-BA 5 mg/L +NAA 0.5 mg/L +AgNO3 2 mg/L + ABA 0.25 mg/L, and the highest frequency of shoot regeneration ( 90.6%) was
achieved on the latter medium. In this research, The determine factors influencing shoot regeneration and strategies to establish efficient regeneration system of Chinese cabbage are discussed.

Key words: Chinese cabbage, Genotype, 6-BA, TDZ, ABA

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