薹菜中硫代葡萄糖苷的鉴定与含量分析

园艺学报 ›› 2008, Vol. 35 ›› Issue (8): 1161-1166.

薹菜中硫代葡萄糖苷的鉴定与含量分析

宋廷宇¹;侯喜林^1*;何启伟^2* ;吴春燕³ ;徐苑芳⁴

（¹南京农业大学作物遗传与种质创新国家重点实验室，南京210095；²山东农业科学院蔬菜研究所，济南 250100；³山东农业大学园艺科学与工程学院，山东泰安271018；⁴济南北方农艺科学研究所，济南 250100）

收稿日期:2008-05-05 修回日期:2008-07-14 出版日期:2008-08-25 发布日期:2008-08-25
通讯作者: 侯喜林;何启伟

Evaluation of Glucosinolate Composition and Contents in Tai-tsai

SONG Ting-yu¹, HOU Xi-lin¹﹡, HE Qi-wei²﹡,WU Chun-yan³,and XU Yuan-fang4

(¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095,China;²Institute of Vegetable ,Shandong Academy of Agricultural Sciences, Jinan 250100,China;³Department of Horticultural Sciences and Engineering, Shandong Agricultural University, Tai'an Shandong 271018,China; ⁴Jinan Northern Institute of Agricultural Sciences, Jinan 250100,China )

Received:2008-05-05 Revised:2008-07-14 Online:2008-08-25 Published:2008-08-25
Contact: HOU Xi-lin;HE Qi-wei

摘要/Abstract

摘要： 采用HPLC的方法，以‘京研薹菜’、‘南京小叶薹菜’、‘花叶薹菜’和‘小叶薹菜’4个品种为试材，在相同的栽培管理条件下对不同品种、不同食用部位（叶片、叶柄）以及不同的生长季节（春季和秋季）薹菜中的硫苷成分和含量进行了研究。4个品种均含有8种硫苷成分，分别为2-羟基-3-丁烯基硫苷、3-丁烯基硫苷、 4-羟基-3-吲哚基甲基硫苷、 4-戊烯基硫苷、2-苯基乙基硫苷、吲哚-3-甲基硫苷、1-甲氧基-3-吲哚基甲基硫苷和 4-甲氧基吲哚-3-甲基硫苷。各品种叶片中的硫苷含量显著高于叶柄；秋季栽培薹菜的硫苷含量高于春季；品种之间所含硫苷成分虽然相同，但含量差异较大。

关键词: 薹菜, 硫代葡萄糖苷, HPLC分析

Abstract: Four Tai-tsai cultivars,eg.‘Jingyan Taicai’, ‘Nanjing Xiaoye Taicai’, ‘Huaye Taicai’and‘Xiaoye Taicai’grown in different season i.e.spring and autumn were used to evaluate glucosinolate composition and contents in leaf blade and petiole of plants by HPLC analysis. The results showed that eight glucosinolate ingredients including Pro，NAP，4OH，GBN，NAS， GBC，NEO and 4ME were detected in all testing materials. It also showed that glucosinolate contents in leaf blade were higher than that in petiole. Moreover, glucosinolate contents in those cultivated in the autumn were higher than that in spring. Great variation of glucosinolate contents were also found among the four cultivars.

Key words: tai-tsai, glucosinolate, HPLC analysis

中图分类号:

S 634.7

宋廷宇;侯喜林;何启伟;吴春燕;徐苑芳. 薹菜中硫代葡萄糖苷的鉴定与含量分析[J]. 园艺学报, 2008, 35(8): 1161-1166.

SONG Ting-yu;HOU Xi-lin﹡;HE Qi-wei﹡;WU Chun-yan;and XU Yuan-fang. Evaluation of Glucosinolate Composition and Contents in Tai-tsai[J]. ACTA HORTICULTURAE SINICA, 2008, 35(8): 1161-1166.

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