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园艺学报 ›› 2002, Vol. 29 ›› Issue (4): 378-380.

• 研究报告 • 上一篇    下一篇

利用几种园艺作物卷须制片鉴定染色体数目的研究

陈劲枫;钱春桃
  

  1. (作物遗传与种质创新国家重点实验室, 南京农业大学蔬菜研究所, 南京210095)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-08-25 发布日期:2002-08-25

Studies on Chromosome Preparations Using Plant Tendril as Source Tissue

Chen Jinfeng and Qian Chuntao   

  1. (State Key Laboratory of Crop Genetics and Germplasm Enhancement , College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

摘要: 以几种园艺作物的卷须为材料研究染色体制片方法, 发现卷须制片预处理和盐酸解离等环节
的要求比根尖制片严格。利用所确立的卷须制片程序, 验证了Cucumis属种间杂交新种Cucumis×hytivus Chen & Kirkbride 的体细胞染色体数为2n = 38 , 表明在根尖不易获取或材料珍贵稀少的情况下, 卷须制片法是研究有卷须类植物染色体数目的有效方法。

关键词: 卷须, 染色体

Abstract: Plant tendrils of several horticulture species were used as tissue source to research the procedure of making-slides for chromosome preparation. The condition required by tendrils was more stringent than that required
by root tips in pretreatment and hydrolyzation. Through the developed procedure , chromosome number of the new species Cucumis ×hytivus Chen & Kirbride was first cytogenetically confirmed to be 2n = 38. When root tips
cannot be obtained easily , this technique is an optimal alternate method for chromosome visualization in tendril-producing species , and especially valuable for some rare genetic germplasm.

Key words: Tendril, Chromosome

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