朱顶红离体培养快速繁殖体系及胚状体发生

园艺学报 ›› 2002, Vol. 29 ›› Issue (3): 285-287.

朱顶红离体培养快速繁殖体系及胚状体发生

张　松¹;达克东^1,2,3;曹辰兴¹;姜璐琰¹;朱瑞芙¹;吴禄平²

(¹ 山东农业大学园艺学院, 泰安 271018;　²沈阳农业大学园艺学院, 沈阳 110161)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-06-25 发布日期:2002-06-25

Rapid Micropropagation System Via in Vitro Culture in Amaryllis vittata and ItsEmbryogenesis

Zhang Song¹;Da Kedong^1,2;Cao Chenxing¹;Jiang Luyan¹;Zhu Ruifu¹;and Wu Luping²

(¹College of Horticulture , Shandong Agricultural University , Tai’an 271018 , China ;　² College of Horticulture , Shenyang Agricultural University, henyang 110161 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-06-25 Published:2002-06-25

摘要/Abstract

摘要： 以朱顶红鳞茎基部鳞片为外植体, 在MS + NAA 1 mg/L + BA 2 mg/L 培养基上愈伤组织的诱导频率最高, 为93. 3 % , 愈伤组织上可以直接生芽, 生芽频率为90. 0 %。NAA 的诱导效果优于2 ,42D , BA 优于KT。愈靠近鳞茎基部愈容易诱导产生愈伤组织和不定芽。愈伤组织继代培养4 次后有胚状体产生。诱导产生的不定芽和胚状体在MS0 培养基上可以100 %生根。生根后的再生植株移栽到蛭石中, 浇MS 无机盐营养液, 成活率达98 %以上。不生根直接将不定芽移栽到消毒蛭石中, 成活率为85 %以上。

关键词: 朱顶红, 鳞茎, 离体培养, 形态发生, 胚状体

Abstract: Effective plant regeneration was obtained on MS medium supplemented with NAA 1 mg/ L and BA 2 mg/ L , using leaves of basal bulb as explants in Amaryllis vittata. The highest callus induction rate was 93. 3 % and shoot induction rate was 90. 0 %. The effect of NAA on morphogenesis was better than 2 ,42D , and that of BA was better than KT. Embryoid was induced after 4 times of subculture of callus. The regenerated shoots and embryoids were rooted on MS mediumwith no hormones. Plantlets with roots were then transplanted into vermiculite sprayed with MS mineral elements once every two days with the survival rate of more than 98 % , or the regenerated shoots with no roots were planted in sterile vermiculite whose survival rate reached more than 85 % in order to save culture time.

Key words: Amaryllis vittata, Bulb, In vitro culture, Morphogenesis, Embryoid

中图分类号:

S 68

张　松;达克东;;曹辰兴;姜璐琰;朱瑞芙;吴禄平. 朱顶红离体培养快速繁殖体系及胚状体发生[J]. 园艺学报, 2002, 29(3): 285-287.

Zhang Song;Da Kedong;Cao Chenxing;Jiang Luyan;Zhu Ruifu;and Wu Luping. Rapid Micropropagation System Via in Vitro Culture in Amaryllis vittata and ItsEmbryogenesis[J]. ACTA HORTICULTURAE SINICA, 2002, 29(3): 285-287.

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