草原龙胆盐胁迫差减文库的构建及分析1

园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1075-1080.

草原龙胆盐胁迫差减文库的构建及分析1

王继刚¹;张坤²;徐启江¹;李玉花^1*

(¹东北林业大学生命科学学院，哈尔滨 150040；²西南林学院学报编辑部，昆明 650224)

收稿日期:2008-04-21 修回日期:2008-06-11 出版日期:2008-07-25 发布日期:2008-07-25
通讯作者: 李玉花

Construction and Analysis of Eustoma grandiflorum Subtracted cDNA library

WANG Ji-gang¹, ZHANG Kun², XU Qi-jiang¹, and LI Yu-hua^1*

(¹College of Life Science, Northeast Forestry University, Harbin 150040, China;²Editorial Department of Academic Journal, Southwest Forestry University, Kunming Yunnan 650224,China)

Received:2008-04-21 Revised:2008-06-11 Online:2008-07-25 Published:2008-07-25
Contact: LI Yu-hua

摘要/Abstract

摘要： 为了研究草原龙胆抗盐性机理，初步获得其抗盐性相关基因，分别以400 mmol·L-1的NaCl及清水处理的草原龙胆叶片mRNA为tester和driver方，利用抑制差减杂交技术构建了cDNA文库。经过测序和与GO数据库序列比对，获得了658条单一序列，其中61条序列分子功能未知，535条序列无同源性，62条序列有显著同源性。此62条序列注释按照功能分为21类。获得了活性氧清除系统基因DHAR、GST、TRX、CAT，转录因子SMT3，调控基因丝氨酸/苏氨酸激酶基因等。结果说明草原龙胆的盐抗性相关基因与数据库中的模式植物基因既有一定的共性，同时绝大部分的序列又无法用已知的基因进行解释。

关键词: 草原龙胆, 抑制差减杂交, 差异基因, 盐胁迫

Abstract: To research its' salt tolerance mechanism and to get the salt tolerance relative genes of Eustoma grandiflorum, a cDNA library was made by suppression subtractive hybridization technique. The leave mRNA of Eustoma grandiflorum treated with 400 mmol·L^-1 NaCl were tester, and that treated with pure water were driver. From the library, we got 658 unique sequences by sequencing and sequence blasting. 61 of the total sequences are functioning unknown, 535 sequences have no homology and 62 sequences have significance homology to the GO sequence library. The 62 sequences can be classified into 21 function categories. And we finally get the reactive oxygen species scavenging genes: DHAR, GST, TRX, CAT, transcription factor SMT3, and serine/threonine protein kinase gene. It indicated that there are general characters between the salt tolerance genes of Eustoma grandiflorum and model plants, and the most of the sequences can not be explained by the known genes.

Key words: Eustoma grandiflorum, suppression subtractive hybridization, differently expressed genes, salt stress

中图分类号:

S 682

王继刚;张坤;徐启江;李玉花. 草原龙胆盐胁迫差减文库的构建及分析1[J]. 园艺学报, 2008, 35(7): 1075-1080.

WANG Ji-gang;ZHANG Kun;XU Qi-jiang;and LI Yu-hua. Construction and Analysis of Eustoma grandiflorum Subtracted cDNA library[J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 1075-1080.

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