叶用莴苣离体培养和植株再生

园艺学报 ›› 2002, Vol. 29 ›› Issue (2): 181-182.

叶用莴苣离体培养和植株再生

朱路英^{1 ,3} ；刘　玲² ；孟祥栋¹；张振贤¹

(¹山东农业大学园艺系, 泰安271018 ;　² 北京市农林科学院蔬菜研究中心, 北京100089 ;　³ 烟台师范学院生物系, 烟台264025)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-04-25 发布日期:2002-04-25

In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce

Zhu Luying^{1 ,3}, Liu Ling² , Meng Xiangdong¹ , and Zhang Zhenxian¹

(¹Department of Horticulture , Shandong Agriculture University , Tai’an 271018 , China ;　²Beijing Vegetable Research Center , Beijing 100089 , China ;　³Department of Biology , Yantai Normal University , Yantai 264025 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

摘要/Abstract

摘要： 以叶用莴苣( Lactuca sativa L. ) 4 个栽培品种的子叶和真叶为材料进行诱导芽分化及生根成苗试验, 从中选出较理想的品种和培养基配方, 建立了我国叶用莴苣栽培品种的快速、高频离体植株再生体系。外植体分化频率可达100 % , 平均每外植体分化不定芽5. 6 个。3 d 苗龄的子叶分化效果最佳。从子叶外植体培养至形成完整小植株仅需30～35 d。

Abstract: An efficient and fast method for shoot differentiation from cotyledon explants of lettuce (Lactuca sativa L. ) was developed. Shoot differentiation rate of lettuce cultivars reached as high as 100 % , each explant produced up to 5 - 6 seedlings. Factors influencing in vitro explant differentiation were studied. The shoot differentiation and enlongation rate were improved by using cotyledon of three days as explant . The regeneration period from lettuce cotyledon explants to a seedling was shortened to 30 - 35 days.

Key words: Lactuca sativa L., Tissue culture, Organogensis

中图分类号:

S 636. 2

朱路英;;刘　玲;孟祥栋;张振贤. 叶用莴苣离体培养和植株再生[J]. 园艺学报, 2002, 29(2): 181-182.

Zhu Luying;;Liu Ling;Meng Xiangdong;and Zhang Zhenxian. In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce[J]. ACTA HORTICULTURAE SINICA, 2002, 29(2): 181-182.

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[2]	李萍萍;胡永光;赵玉国;毛罕平. 叶用莴苣温室栽培单株光合作用日变化规律[J]. 园艺学报, 2001, 28(3): 240-245.
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