豇豆种子萌发过程中多胺氧化酶活性的变化及其影响因素

园艺学报 ›› 2002, Vol. 29 ›› Issue (2): 153-157.

豇豆种子萌发过程中多胺氧化酶活性的变化及其影响因素

何生根¹；黄学林²；傅家瑞²

(¹ 仲恺农业技术学院, 广州510225 ;　² 中山大学生命科学学院, 广州510275)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-04-25 发布日期:2002-04-25

Change of Polyamine Oxidase Activity during Cowpea Seed Germination and Its Influencing Factors

He Shenggen¹ , Huang Xuelin² , and Fu Jiarui²

(¹ Zhongkai Agrotechnical College , Guangzhou 510225 , China ;　² The School of Life Sciences , Zhongshan University , Guangzhou 510275 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

摘要/Abstract

摘要： 吸胀而未萌发的豇豆( Vigna unguiculata L. ) 种子检测不到多胺氧化酶(PAO) 活性, 直至萌发开始(吸胀后36 h) 才在胚芽、子叶和胚轴测到PAO 活性, 此后各部分的PAO 活性以不同速度增加, 而胚根一直没能检测到PAO 活性。蛋白质合成抑制剂环己酰亚胺(CHM) 吸胀处理可显著抑制萌发种子胚芽和子叶的PAO 活性, 同时强烈抑制种子的萌发; 核酸合成抑制剂放线菌素D 处理对豇豆胚芽和子叶的PAO活性以及种子萌发的影响则远不及CHM处理。豇豆种子在黑暗条件下萌发, 胚芽和子叶的PAO 活性都明显高于光下。适宜浓度的腐胺和CuCl₂ 吸胀处理也可提高胚芽和子叶的PAO 活性。

关键词: 豇豆, 多胺, 多胺氧化酶, 种子萌发, Cu²⁺

Abstract: No PAO activity was detected in ungerminated cowpea (Vigna unguiculata L. ) seeds (form 0 to 24 h after imbibition) , but it could be detected in the plumule , cotyledons and embryonic axis of the germinatingseeds whose radicles were just visible (36 h after imbibition) . The PAO activities in these three parts increased at different rates during germination , while no PAO could be detected in radicle at all times. Soaking with Act2D only inhibited the seed germination by 27. 6 % , but CHM could inhibit the germination by 90. 6 %. Moreover , the inhibitions of the PAO activity in the plumule and cotyledons by Act2D were just 29. 1 % and 33. 3 % , and those by CHM were up to 87. 2 % and 85. 4 % respectively. Light had a remarkable inhibitory effect on the PAO activity in plumule and cotyledons. Soaking with putrecine (0. 1 and 0. 5 mmol/ L) or CuCl₂ (10μmol/ L) for 6 h could enhance the activity of PAO.

Key words: Cowpea ( Vigna unguiculata L. ), Polyamine, Polyamine oxidase, Seed germination, Cu²⁺

中图分类号:

何生根;黄学林;傅家瑞. 豇豆种子萌发过程中多胺氧化酶活性的变化及其影响因素[J]. 园艺学报, 2002, 29(2): 153-157.

He Shenggen;Huang Xuelin;and Fu Jiarui. Change of Polyamine Oxidase Activity during Cowpea Seed Germination and Its Influencing Factors[J]. ACTA HORTICULTURAE SINICA, 2002, 29(2): 153-157.

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