利用RAPD分析鉴别荔枝的焦核突变体

园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 57-59.

利用RAPD分析鉴别荔枝的焦核突变体

刘成明^{1 ,2};梅曼彤²

(¹ 华南农业大学园艺系, 广州510642 ;　² 华南农业大学遗传工程研究室, 广州510642)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

Identification of Stenospermocarpy Mutants of Litchi by RAPD Analysis

Liu Chengming^{1 ,2} and Mei Mantong²

(¹ Department of Horticulture South China Agricultural University, Guangzhou 510642;　² Genetic Engineering Laboratory , South China Agricultural University, Guangzhou 510642)

Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要/Abstract

摘要： 采用随机扩增多态性DNA 方法, 对荔枝品种三月红、怀枝和它们的9 个自然焦核突变体进行
了多态性分析。经500 个随机引物的筛选, 发现仅1 个引物OPL212 能在品种与突变体间扩增出多态性片段,其中2个焦核怀枝的特异片段大小为1 645 bp, 4 个焦核三月红的特异片段大小为722 bp , 初步认为这两条特异片段可能与荔枝的焦核基因相关。

关键词: 荔枝, 焦核突变体, RAPD

Abstract: For understanding the mechanismof stenospermocarpy mutation in litchi (Litchi chinensis Sonn. ) , RAPD analysis was conducted in this study. Huaizhi and Sanyuehong , two cultivars grown in South China, and nine spontaneous mutants from them were used as materials. After screening 500 102mer random primers for polymor phism among these two cultivars and their mutants , only one prime , OPL212 , was able to generate different elec
trophoresis pattern between Huaizhi and its two seed2abortive mutants , as well as Sanyuehong and its four seed abortive mutants. The size of amplified fragments specifically for seed2abortive Huaizhi mutants was 1 645 bp , and
which for seed2abortive Sanyuehong was 722 bp. The two specific amplified bands had been considered as markers of seed abortion genes in litchi.

Key words: Litchi, Seed2abortive mutant, RAPD

中图分类号:

S 667. 1

刘成明;梅曼彤. 利用RAPD分析鉴别荔枝的焦核突变体[J]. 园艺学报, 2002, 29(1): 57-59.

Liu Chengming;and Mei Mantong. Identification of Stenospermocarpy Mutants of Litchi by RAPD Analysis[J]. ACTA HORTICULTURAE SINICA, 2002, 29(1): 57-59.

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