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园艺学报 ›› 2011, Vol. 38 ›› Issue (1): 95-100.

• 蔬菜 • 上一篇    下一篇

南瓜矮生基因Bu的比较定位

王深浩1,*,李海真2,*,张忠华1,贺 俊1,贾长才2,张 帆2,黄三文1,**   

  1. (1中国农业科学院蔬菜花卉研究所,农业部园艺作物遗传改良重点实验室,中荷联合园艺作物基因组技术实验室,北京100081;2北京市农林科学院蔬菜研究中心,北京100097)
  • 收稿日期:2010-06-03 修回日期:2011-08-01 出版日期:2011-01-25 发布日期:2011-01-25
  • 通讯作者: 黄三文

Comparative Mapping of the Dwarf Gene Bu from Tropical Pumpkin (Cucurbita moschata Duchesne)

WANG Shen-hao1,*,LI Hai-zhen2,*,ZHANG Zhong-hua1,HE Jun1,JIA Chang-cai2,ZHANG Fan2,and HUANG San-wen1,**   

  1. (1Key Laboratory of Horticultural Crops Genetic Improvement of Ministry of Agriculture,Sino-Dutch Joint Lab of Horticultural Genomics Technology,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
  • Received:2010-06-03 Revised:2011-08-01 Online:2011-01-25 Published:2011-01-25
  • Contact: HUANG San-wen

摘要: 以中国南瓜矮生突变体为供体亲本,以印度蔓生南瓜为轮回亲本,构建了BC6F2分离群体。利用黄瓜基因组序列,将南瓜矮生基因Bu比较定位至黄瓜5号染色体,并开发了一个新的PCR标记IF3629,该标记与矮生基因Bu连锁遗传距离为1.0 cM。该标记不仅可以用于分子标记辅助选择育种,而且为Bu基因的克隆奠定了基础。

关键词: 南瓜, 矮生基因, 比较基因组学, 分子标记

Abstract: A segregating BC6F2 population was developed using a dwarf mutant of Cucurbita moschata Duch. as donor and the vine C. maxima Duch.‘Mengri’as recurrent parent. Using the segregating population,the gene Bu was comparatively mapped on chromosome 5 of cucumber. An intron-flanking marker IF3629 was found to be tightly linked to the Bu gene,with a genetic distance of 1.0 cM. The marker IF3629 will not only be useful in markers-assisted selection for dwarf plant,but also provide a starting point toward positional isolation of the dwarf Bu gene in squash.

Key words: squash, dwarf gene, comparative genomics, molecular marker

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