高温胁迫下番茄叶片差异表达基因的cDNA-AFLP鉴定

园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1011-1016.

高温胁迫下番茄叶片差异表达基因的cDNA-AFLP鉴定

刘志勇^1,2;杜永臣^1*;王孝宣¹;国艳梅¹;高建昌¹

（¹中国农业科学院蔬菜花卉研究所，北京 100081;²上海市农业科学院园艺所，上海 201106）

收稿日期:2008-03-05 修回日期:2008-05-26 出版日期:2008-07-25 发布日期:2008-07-25
通讯作者: 杜永臣

Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves

LIU Zhi-yong^1,2, DU Yong-chen^1*, WANG Xiao-xuan¹, GUO Yan-mei¹, and GAO Jian-chang¹

（¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;²Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China）

Received:2008-03-05 Revised:2008-05-26 Online:2008-07-25 Published:2008-07-25
Contact: DU Yong-chen

摘要/Abstract

摘要： 为鉴定番茄高温胁迫响应基因，应用cDNA-AFLP技术，以番茄耐热品系Saladette幼苗为研究对象，对高温胁迫早期叶片基因表达进行了mRNA指纹分析。通过768对引物组合的筛选，共分离得到187个差异表达的转录衍生片段(TDF)，其中增强表达或高温胁迫下特异性表达TDF153条，抑制表达34条。对其中47个TDF进行了克隆、测序和序列分析。结果表明：34个TDF与NCBI或TIGR已有序列同源（E-Value<10^-5），功能涉及信号传导、转录调控以及逆境诱导蛋白等，另有13个TDF无同源序列或同源性低，预测是一些未知功能基因。

关键词: 番茄, 高温胁迫, cDNA-AFLP, 差异表达基因

Abstract: In order to identify genes involved in heat shock responses in tomato, cDNA-AFLP approach was employed to identify genes differentially expressed in tomato leaves at high temperature. 768 primer combinations were used for selective amplification. 187 TDFs were selected for their differential expression under heat stress. Among the 187 TDFs, 153 TDFs were up-regulated and 34 TDFs were suppressed. 47 TDFs were cloned and sequenced. Compared with the publicly available databases, 34 TDFs presented some significant similarity with known plant sequences, whose functions are involved in gene regulation, transcription factor, signal transduction, stress defense，etc. 13 TDFs with low identity and no match to known genes may represent novel tomato genes.

Key words: tomato (Solanum lycopersicum), heat stress, cDNA-AFLP, differentially expressed genes

中图分类号:

S 641.2

刘志勇;杜永臣;王孝宣;国艳梅;高建昌 . 高温胁迫下番茄叶片差异表达基因的cDNA-AFLP鉴定[J]. 园艺学报, 2008, 35(7): 1011-1016.

LIU Zhi-yong;DU Yong-chen;WANG Xiao-xuan;GUO Yan-mei;and GAO Jian-chang. Identification of Differentially Expressed Genes by cDNA-AFLP Approach During Heat Stress in Tomato Leaves[J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 1011-1016.

[1]	史洪丽, 李腊, 郭翠梅, 余婷婷, 简伟, 杨星勇. 番茄灰霉病生防菌株TL1的分离、鉴定及其生防能力分析[J]. 园艺学报, 2023, 50(1): 79-90.
[2]	忽靖宇, 阙开娟, 缪田丽, 吴少政, 王田田, 张磊, 董鲜, 季鹏章, 董家红. 侵染鸢尾的番茄斑萎病毒鉴定[J]. 园艺学报, 2023, 50(1): 170-176.
[3]	郑积荣, 王同林, 胡松申. 高品质番茄新品种‘杭杂603’[J]. 园艺学报, 2022, 49(S2): 103-104.
[4]	郑积荣, 王同林. 番茄新品种‘杭杂601’[J]. 园艺学报, 2022, 49(S2): 105-106.
[5]	郑积荣, 王同林. 樱桃番茄新品种‘杭杂503’[J]. 园艺学报, 2022, 49(S2): 107-108.
[6]	黄婷婷, 刘淑芹, 张永志, 李平, 张志焕, 宋立波. 樱桃番茄新品种‘樱莎红4号’[J]. 园艺学报, 2022, 49(S2): 109-110.
[7]	张前荣, 李大忠, 裘波音, 林珲, 马慧斐, 叶新如, 刘建汀, 朱海生, 温庆放. 设施番茄新品种‘闽农科2号’[J]. 园艺学报, 2022, 49(S1): 73-74.
[8]	侯潞丹, 赵梦然, 黄晨阳, 张金霞. 交替氧化酶增强糙皮侧耳高温耐受性的分子机制[J]. 园艺学报, 2022, 49(9): 1922-1934.
[9]	韩帅, 吴婕, 张河庆, 席亚东. 四川莴笋上番茄斑萎病毒的电镜观察与小RNA测序鉴定[J]. 园艺学报, 2022, 49(9): 2007-2016.
[10]	陈礼浪, 杨天章, 蔡儒平, 林小漫, 邓南康, 车海彦, 林雅婷, 孔祥义. 海南西番莲主要病毒种类的分子检测与鉴定[J]. 园艺学报, 2022, 49(8): 1785-1794.
[11]	路涛, 余宏军, 李强, 蒋卫杰. 叶果量调控对番茄生长发育、果实品质和产量的影响[J]. 园艺学报, 2022, 49(6): 1261-1274.
[12]	孟宪敏, 崔青青, 段韫丹, 庄团结, 濮丹, 董春娟, 杨文才, 尚庆茂. 烯效唑对番茄幼苗嫁接愈合的促进作用及其机理研究[J]. 园艺学报, 2022, 49(6): 1275-1289.
[13]	刘尚佳, 吕尧, 曹逼力, 陈子敬, 高松, 徐坤. 高温渍涝胁迫对姜叶片光合作用和氮代谢的影响[J]. 园艺学报, 2022, 49(5): 1073-1080.
[14]	崔东禹, 李长青, 孙焱鑫, 王激清, 邹国元, 杨俊刚. 温室番茄东西向栽培条件下矮化密植对其生长和产量的影响[J]. 园艺学报, 2022, 49(4): 875-884.
[15]	陈同强, 张天柱, 王晓卓. 光照对番茄果实中番茄红素生物合成的调控研究进展[J]. 园艺学报, 2022, 49(4): 907-923.