花椰菜叶绿素荧光参数与耐寒性的关系研究

园艺学报 ›› 2010, Vol. 37 ›› Issue (12): 2001-2001–2006.

花椰菜叶绿素荧光参数与耐寒性的关系研究

李光庆1,2，谢祝捷1,*，姚雪琴1，陈学好2

（1上海市农业科学院园艺研究所，上海市设施园艺技术重点实验室，上海 201106；2扬州大学园艺与植物保护学院，江苏扬州 225009）

收稿日期:2010-05-21 修回日期:2010-10-08 出版日期:2010-12-25 发布日期:2010-12-25
通讯作者: 谢祝捷

Studies on the Relationship Between Chlorophyll Fluorescence Parameters and Cold Tolerance of Cauliflower

LI Guang-qing1,2，XIE Zhu-jie1,*，YAO Xue-qin1，and CHEN Xue-hao2

（1Institute of Horticulture，Shanghai Academy of Agricultural Sciences，Shanghai Key Laboratory of Protected Horticultural Technology，Shanghai 201106，China；2College of Horticulture and Planting Protection，Yangzhou University，Yangzhou，Jiangsu 225009，China）

Received:2010-05-21 Revised:2010-10-08 Online:2010-12-25 Published:2010-12-25
Contact: XIE Zhu-jie

摘要/Abstract

摘要： 以7个不同基因型花椰菜品种为材料，研究了低温胁迫对不同熟性和相同熟性花椰菜生长指标、冻害指数和叶绿素荧光参数的影响，以及相同熟性花椰菜冻害前后叶绿素荧光参数的变化，分析了花椰菜生长、耐寒性和叶绿素荧光参数的相关性。结果表明：低温胁迫下，冻害指数和叶绿素荧光参数中的初始荧光（Fo）仅在不同熟性的品种间具有显著差异，花球质量、植株营养体质量以及叶绿素相对含量（SPAD）和叶绿素荧光参数中的原初光能转化效率（Fv/Fm）在不同熟性和相同熟性的品种间均具有显著差异。相同熟性品种间在冻害前后Fv/Fm值均有显著差异。对不同基因型的生长指标、冻害指数和叶绿素荧光参数间的相关分析表明，直接反映花椰菜耐寒性的冻害指数与SPAD值和叶绿素荧光参数Fv/Fm值均显著负相关；间接反映花椰菜耐寒性的生长指标花球质量和营养体质量与叶绿素荧光参数Fv/Fm值显著正相关，而生长指标与SPAD值仅在不同熟性花椰菜间显著正相关。光化学转化效率与花椰菜的耐寒性紧密相关，叶绿素荧光参数Fv/Fm值可能为不同基因型间花椰菜耐寒性的鉴定指标。

关键词: 花椰菜, 耐寒性鉴定, 叶绿素荧光参数, 低温胁迫

Abstract: The effects of cold stress on growth index，freezing injury index and chlorophyll fluorescence parameters in seven different genotype cauliflowers with different or same maturity were studied. The changes of chlorophyll fluorescence parameters in different genotype cauliflowers with same maturity under freezing injury stress were also examined. The correlations among growth，cold tolerance and chlorophyll fluorescence parameters were analyzed in cauliflower varieties with different or same maturity. The results showed that there were significant differences among the varieties with different maturity in freezing injury index and minimal fluorescence（Fo），and there were significant differences among the varieties with the same and different maturity in head weight，vegetative mass weight，SPAD value and conversion efficiency of primary light energy（Fv/Fm）. Significant differences existed among the varieties with same maturity under freezing injury stress. Correlation analysis of growth index，freezing injury index and chlorophyll fluorescence parameters in different genotype varieties showed that freezing injury index which directly reflects cold tolerance were negatively correlated with SPAD and Fv/Fm. Head weight and vegetative mass weight which indirectly but externally reflects cold tolerance，were positively correlated with Fv/Fm in varieties with both same and different maturity，and positively correlated with SPAD only in varieties with different maturity. In conclusion，conversion efficiency of primary light energy closely related to cold tolerance，Fv/Fm could be used as index of cold tolerance identification in cauliflowers with both same and different maturity.

Key words: cauliflower, cold tolerance identification, chlorophyll fluorescence parameters, cold stress

中图分类号:

S 635

李光庆;谢祝捷;姚雪琴;陈学好. 花椰菜叶绿素荧光参数与耐寒性的关系研究[J]. 园艺学报, 2010, 37(12): 2001-2001–2006.

LI Guang-qing;XIE Zhu-jie;YAO Xue-qin;and CHEN Xue-hao. Studies on the Relationship Between Chlorophyll Fluorescence Parameters and Cold Tolerance of Cauliflower[J]. ACTA HORTICULTURAE SINICA, 2010, 37(12): 2001-2001–2006.

[1]	任菲, 卢苗苗, 刘吉祥, 陈信立, 刘道凤, 眭顺照, 马婧. 蜡梅胚胎晚期丰富蛋白基因CpLEA的表达及抗性分析[J]. 园艺学报, 2023, 50(2): 359-370.
[2]	王梦梦, 孙德岭, 陈锐, 杨迎霞, 张冠, 吕明杰, 王倩, 谢添羽, 牛国保, 单晓政, 谭津, 姚星伟, . 花椰菜核心种质的构建与评价[J]. 园艺学报, 2023, 50(2): 421-431.
[3]	蔺海娇, 梁雨晨, 李玲, 马军, 张璐, 兰振颖, 苑泽宁. 薰衣草CBF途径相关耐寒基因挖掘与调控网络分析[J]. 园艺学报, 2023, 50(1): 131-144.
[4]	李琼, 李丽丽, 侯娟, 罗忍忍, 王瑞丹, 胡建斌, 黄松. 瓜类作物响应低温胁迫机制的研究进展[J]. 园艺学报, 2022, 49(6): 1382-1394.
[5]	王光鹏, 刘同坤, 徐新凤, 李竹帛, 高瞻远, 侯喜林. 大白菜LEA家族基因的鉴定及其部分成员在低温胁迫下的表达分析[J]. 园艺学报, 2022, 49(2): 304-318.
[6]	朱世杨, 张小玲, 刘庆, 钟伟杰, 罗天宽, 唐征, 徐谦, 周元昌. 花椰菜不同胞质来源雄性不育的细胞质效应分析[J]. 园艺学报, 2021, 48(6): 1107-1122.
[7]	俞沁含, 焦淑珍, 吴楠, 张宁波, 徐伟荣. 葡萄E3泛素酶HOS1基因克隆、表达及抗血清制备[J]. 园艺学报, 2021, 48(6): 1173-1180.
[8]	张小玲, 刘庆, 朱世杨, 钟伟杰, 罗天宽, 唐征, 裘波音. 松散型花椰菜新品种‘瓯松60天’[J]. 园艺学报, 2020, 47(9): 1861-1862.
[9]	刘彦英，倪珊珊，项蕾蕾，陈裕坤，赖钟雄*. 香蕉漆酶基因家族鉴定及低温胁迫下的表达分析[J]. 园艺学报, 2020, 47(5): 837-852.
[10]	付亚娟 1,2，陈霞婷 1，乔洁 1,2，王晶 1,2，李文静 1，侯晓强 1,2,*. 铁皮石斛亲环蛋白基因 DoCyP 的克隆及表达分析[J]. 园艺学报, 2020, 47(3): 581-589.
[11]	刘丰娇，张晓伟，李福德，翟江，毕焕改，艾希珍. 黄瓜富氢水浸种对低温下幼苗光合碳同化及氮代谢的影响[J]. 园艺学报, 2020, 47(2): 287-300.
[12]	郑巧玲, 申威, 姚文孔, 徐伟荣, . 山葡萄低温诱导酵母双杂三框cDNA文库构建和VaCIPK18互作蛋白筛选鉴定[J]. 园艺学报, 2020, 47(12): 2301-2316.
[13]	崔波1,*，郝平安2，梁芳1，张燕1，王喜蒙1,4，李俊霖1,3，蒋素华1，许申平1. 蝴蝶兰AP2/ERF家族基因的克隆及在低温下表达特性分析[J]. 园艺学报, 2020, 47(1): 85-97.
[14]	姚星伟，牛国保，刘莉莉，单晓政，文正华，江汉民，张小丽，孙德岭*. 花椰菜新品种‘津品75’[J]. 园艺学报, 2019, 46(S2): 2783-2784.
[15]	刘庆，张小玲*，朱世杨，唐征，裘波音，罗天宽. 松散型花椰菜新品种‘瓯松90天’[J]. 园艺学报, 2019, 46(S2): 2785-2786.