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园艺学报 ›› 2010, Vol. 37 ›› Issue (9): 1439-1448.

• 蔬菜 • 上一篇    下一篇

适度水分胁迫提高黄瓜幼苗光合作用弱光适应性

肖文静1,孙建磊1,王绍辉1,2,孙惠慧1,王虹云1,眭晓蕾1,*,张振贤1,*   

  1. (1中国农业大学农学与生物技术学院,北京 100193;2北京农学院植物科学技术系,北京 102206)
  • 收稿日期:2010-04-08 修回日期:2010-07-15 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 眭晓蕾,张振贤

Moderate Water Stress Increased the Photosynthesis Adaptability of Cucumber Seedlings Under Low Light

XIAO Wen-jing1,SUN Jian-lei1,WANG Shao-hui1,2,SUN Hui-hui1,WANG Hong-yun1,SUI Xiao-lei1,*,and ZHANG Zhen-xian1,*   

  1. (1College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China;2Department of Plant Science & Technology,Beijing University of Agriculture,Beijing 102206,China)
  • Received:2010-04-08 Revised:2010-07-15 Online:2010-09-25 Published:2010-09-25
  • Contact: SUI Xiao-lei, ZHANG Zhen-xian

摘要: 以黄瓜(Cucumis sativus L.)‘戴多星’品种为试材,在人工气候室内研究弱光(75 ~ 85 μmol · m-2 · s-1)下水分胁迫(40%基质最大持水量)对幼苗叶片光合、荧光特性及Rubisco含量的影响。结果表明,弱光逆境下,经20 d水分胁迫后的幼苗较正常水分条件下叶片光合色素(以单位面积计)含量提高,叶绿素a/b(Chl.a/b)下降,植株光饱和光合速率Asat、表观量子效率(AQY)、羧化效率(CE)、RuBP最大再生速率及光饱和点(LSP)均显著增加,CO2补偿点(CCP)显著下降,并且光系统Ⅱ(PSⅡ)最大光化学效率(Fv/Fm)、潜在光化学效率(Fv/Fo)、PSⅡ光化学量子效率(ΦPSⅡ)、光化学反应速率(Prate)、非环式电子传递速率(J)以及PSⅡ吸收光能向光化学反应分配比例P等叶绿素荧光参数呈增加趋势,PSⅠ和PSⅡ间激发能分配不平衡性(β / α–1)减小,叶片可溶性蛋白质含量和Rubisco大、小亚基含量上升,表明适度的水分胁迫有利于提高弱光下黄瓜幼苗的光合适应性;而正常光照下(500 ~ 600 μmol · m-2 · s-1)经水分胁迫后的黄瓜幼苗上述主要光合特性指标如Asat、RuBP最大再生速率、CE、ΦPSⅡ、Prate、J和P等有所下降,光系统间激发能分配不平衡性(β / α–1)增加,光合作用受到一定抑制。

关键词: 黄瓜, 弱光, 水分胁迫, 光合特性, 5–二磷酸核酮糖羧化/加氧酶(Rubisco)

Abstract: The effects of water stress(40% maximum moisture capacity)on the photosynthetic and fluorescent characteristics and the content of Rubisco of Cucumis sativus‘Deltastar’seedlings under low light(75–85 μmol · m-2 · s-1)were investigated in a phytotron. The results revealed that after 20 days of water stress under low light,the photosynthesis pigment contents per unit area of leaves of cucumber increased and the values of Chl.a/b decreased. The values of light saturated rate of the CO2 assimilation(Asat),apparent quantum yield(AQY),carboxylation efficiency(CE),maximum RuBP regeneration rate,light saturation point(LSP)increased significantly but CO2 compensation point(CCP)decreased remarkably. The chlorophyll fluorescence parameters including PSⅡ maximal photochemical efficiency(Fv/Fm),PSⅡ potential photochemical efficiency(Fv/Fo),PSⅡ actual photochemical efficiency(ΦPSⅡ),the photochemical reaction rate(Prate),the linear whole-chain electron transport rate(J)and the partitioning proportion for photochemical reaction of light energy absorbed by PSⅡ(P)showed increased trend,but the imbalance of partitioning of excitation energy between PSⅠand PSⅡ(β/α–1) decreased. The content of soluble protein and the content of Rubisco increased. These results indicated moderate water stress increased the photosynthesis adaptability of cucumber seedlings under low light. However,after 20 days of water stress under normal light(500–600 μmol · m-2 · s-1),the values of the items including Asat,maximum RuBP regeneration rate,CE,ΦPSⅡ,Prate and P decreased. But the values of(β/α–1) increased. So the photosynthesis of cucumber under normal light was inhibited by water stress.

Key words: cucumber, low light, water stress, photosynthetic characteristics, Ribulose-1, 5-bisphosphate carboxylase/oxygenase(Rubisco)

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