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园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1585-1588.

• 新技术新品种 • 上一篇    下一篇

应用大孔吸附树脂分离纯化香椿叶总黄酮

张京芳,王冬梅*   

  1. (西北农林科技大学林学院,陕西 杨凌 712100)
  • 收稿日期:2007-06-25 修回日期:2007-09-12 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 王冬梅

Separation and Purification of Total Flavonoids From Toona sinensis Leaves by Macroporous Resins

ZHANG Jing-fang and WANG Dong-mei*   

  1. (College of Forest, Northwest A &F University,Yangling,Shaanxi 712100,China)
  • Received:2007-06-25 Revised:2007-09-12 Online:2007-12-25 Published:2007-12-25

摘要: 测定了6种大孔吸附树脂对香椿叶中总黄酮的吸附解吸性能,确定了大孔吸附树脂吸附分离香椿叶总黄酮的工艺条件。结果表明LSA-10树脂对香椿叶总黄酮有良好的吸附解吸性能,其最佳吸附分离的工艺条件为:上柱液总黄酮质量浓度0.653 mg/mL,pH 4.0~5.5,流速240 mL /h,饱和吸附量440~480 mL,用400 mL 50%乙醇以流速80 mL /h解吸。经4次上柱吸附解吸,香椿叶总黄酮纯度可达75.2%。

关键词: 香椿, 叶, 总黄酮, 大孔吸附树脂, 分离纯化

Abstract: In order to establish the technology for separating and purifying total flavonoids from Toona sinensis leaves with macroporous resin, 6 types of macroporous resins were selected to be investigated. The results showed that LSA-10 was the best for separating and purifying total flavonoids in the following conditions: the loaded concentration of total flavones 0.653 mg/mL, pH4.0-5.5, the flow rate 240 mL/h; amount of saturated adsorption for total flavones 440-480mL; 400 mL 50% ethanol as appropriate the eluting reagent, eluting rate 80 mL /h. The purity of total flavonoids purified by LSA-10 was 75.2% when purified four times under the optimal conditions.

Key words: Toona sinensis, Leaf, Total flavonoid, Macroporous resin, Separation, Purification

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