白玉凤尾蕨孢子繁殖技术的研究

园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 658-662.

白玉凤尾蕨孢子繁殖技术的研究

徐艳1, 2;石雷1;刘燕2;李东1

(¹中国科学院植物研究所, 北京100093; ² 北京林业大学园林学院, 北京100083)

收稿日期:2004-09-27 修回日期:2005-01-15 出版日期:2005-08-25 发布日期:2005-08-25

Studies on Spore Propagation of Pteris cretica ‘Albo-lineata’

Xu Yan1, 2;Shi Lei1;Liu Yan2;Li Dong1

(¹ Institute of Botany, the Chinese Academy of Science, Beijng 100093, China; ²College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China)

Received:2004-09-27 Revised:2005-01-15 Online:2005-08-25 Published:2005-08-25

摘要/Abstract

摘要： 研究了白玉凤尾蕨( Pteris cretica ‘Albo-lineata’) 孢子的无菌培养和常规繁殖方法。结果表明: 孢子萌发为书带蕨型, 配子体发育为水蕨型。1 /2MS培养基中萌发率最高, 可达82.3%; 低于2%的蔗糖浓度较适于孢子的萌发; MS培养基中配子体成苗率最高, 可达53.4%; 试管苗移栽成活率可达80%以上。草炭土+河沙(体积比1∶1) 是播种孢子的理想基质, 配子体成苗率达8912%; 当幼孢子体苗的第1片子叶长至1～2 cm长时分苗, 移栽到草炭土+蛭石+素砂+有机肥(体积比4∶2∶2∶1) 的栽培基质上,移栽成活率最高, 可达98.1%。幼孢子体苗长至10 cm左右时已经具有一定的观赏价值。

关键词: 凤尾蕨, 孢子, 无菌培养, 常规繁殖

Abstract: The spore sterile culture and routine propagation of Pteris cretica ‘Albo-lineata’were studied respectively. The results showed that the spore germination was of the Vittaria2type while the gametophyte development was of the Ceratop teris2type. The maximum germination rate was obtained in 1 /2MS medium, which reached 82.3%; the medium with sucrose concentration being less than 2% was appropriate for spore germination; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached
53.4%; the transplant survival rate of sporophyte in vitro reached above 80%. The mixed soil ( the turf and the sand blend in the volume ratio of 1 to 1) was the optimal growing medium for spore routine propagation, in which the rate of gametophyte to the sporophyte was up to 89.2%. When the first cotyledon of the sporophyte seedling grew to 1 - 2 cm, the sporophyte seedlings should be rep lanted in the growing medium which was composed of turf, vermiculite, sand and organic fertilizers. The highest survival rate reached 98.1%. The
young gametophyte seedlings of 10 cm height had already possessed ornamental values.

Key words: Pteris cretica, Spore, Routine propagation, Sterile culture

中图分类号:

S 682.35

徐艳;;石雷;刘燕;李东. 白玉凤尾蕨孢子繁殖技术的研究[J]. 园艺学报, 2005, 32(4): 658-662.

Xu Yan;;Shi Lei;Liu Yan;Li Dong. Studies on Spore Propagation of Pteris cretica ‘Albo-lineata’[J]. ACTA HORTICULTURAE SINICA, 2005, 32(4): 658-662.

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