苦瓜自交系多糖结构组成的比较分析

园艺学报 ›› 2010, Vol. 37 ›› Issue (7): 1169-1174.

苦瓜自交系多糖结构组成的比较分析

张德1，向长萍1,*，李俊丽2，孔令伟3

（1华中农业大学园艺林学学院，国家蔬菜改良中心华中分中心，园艺植物生物学教育部重点实验室，武汉 430070；2武汉理工大学化学工程学院，武汉 430070；3南昌大学，食品科学与技术国家重点实验室，南昌 330047）

收稿日期:2010-01-25 修回日期:2010-03-24 出版日期:2010-07-25 发布日期:2010-07-25
通讯作者: 向长萍

Comparative Analysis of Polysaccharides Structure and Composition in Bitter Melon Inbreed Lines

ZHANG De1，XIANG Chang-ping1,*，LI Jun-li2，and KONG Ling-wei3

（1College of Horticulture and Forestry Sciences，Huazhong Agriculture University，Huazhong Branch of National Vegetable Improvement Center，the Ministry of Education Key Laboratory of Horticultural Plant Biology，Wuhan 430070，China；2Department of Chemical Engineering，Wuhan University of Technology，Wuhan 430070，China；3State Key Laboratory of Food Science and Technology，Nanchang University，Nanchang 330047，China）

Received:2010-01-25 Revised:2010-03-24 Online:2010-07-25 Published:2010-07-25
Contact: XIANG Chang-ping

摘要/Abstract

摘要： 对苦瓜3个自交系果实中的多糖结构和组成进行了比较研究。采用热水浸提乙醇沉淀法得到苦瓜粗多糖，进一步经过脱蛋白、透析等纯化工艺得到苦瓜多糖，应用气相色谱法测定其多糖组成，红外光谱法测定其糖苷键类型。研究结果表明：在同一生长环境下，3个苦瓜自交系多糖在其含量、单糖组成以及结构上均存在差异；苦瓜水溶性多糖主要由阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，此外3号和8号自交系还含有少量的鼠李糖和木糖；构型分析表明苦瓜多糖主要以α型和β型吡喃糖形式存在。

关键词: 苦瓜, 苦瓜多糖, 单糖组成, 红外光谱, 气相色谱

Abstract: The polysaccharides structure and composition of fruit in three bitter melon inbred lines were compared in the paper. The crude polysaccharides were extracted in hot water and precipitated with alcohol. Then，the polysacchrides were purified by the processes of protein removing and dialysis. The polysaccharides composition and the types of its glycosidic bond were determined by gas chromatography and infrared spectroscopy respectively. The results showed that in the same growing environment，there are significant differences in the polysaccharide content，monosaccharide composition and the structure among different bitter melon inbreed lines. The water-soluble polysaccharide of bitter melon is mainly composed of arabinose，mannose，glucose and galactose，with the exception that inbreed lines of No.3 and No.8 contain small amount of rhamnose and xylose. In addition，configuration analysis showed the polysaccharides mainly exist in the forms of α- and β-pyranose in bitter melon.

Key words: bitter melon, bitter melon polysaccharide, monosaccharide composition, infrared spectroscopy, gas chromatography

中图分类号:

S 642.5

张德;向长萍;李俊丽;孔令伟. 苦瓜自交系多糖结构组成的比较分析[J]. 园艺学报, 2010, 37(7): 1169-1174.

ZHANG De;XIANG Chang-ping;LI Jun-li;and KONG Ling-wei. Comparative Analysis of Polysaccharides Structure and Composition in Bitter Melon Inbreed Lines[J]. ACTA HORTICULTURAE SINICA, 2010, 37(7): 1169-1174.

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