关键词: 番茄, 醋栗番茄, 耐冷性, 高代回交QTL分析

Abstract: An advanced backcross population of 273 BC3S1 individuals was developed derived from the cross Solanum pimpinellifolium LA722 × Solanum lycopersicum 9706 and a map was constructed containing 35 CAPS markers and 62 SSR markers. The QTLs controlling cold tolerance were mapped in the wild tomato Solanum pimpinellifolium LA722 by mass screening test under cold stress. Using MQM mapping method, seven QTLs controlling cold tolerance were mapped in five chromosomes. The five QTLs coffering cold-tolerance during seed germination were mapped to chromosomes 1, 2, 5 and 11, while the two QTLs coffering cold-tolerance during seedling stage were mapped to chromosome 2 and 8. The QTLs on chromosomes 2 and 5 (qRGI-2-1 and qRGI-5-1) could explain 12.8% and 32.9% of the phenotypic variance, respectively. The cold tolerance of 12 tomato self-offspring (BC3S2) during seed germination and seedling stages was at or better than that of LA722 and the agronomic traits, such as fruit weight, were also close to or better than normal tomato variety 9706. So these lines can be used as tomatocold-resistant breeding materials.

Key words: tomato, Solanum pimpinellifolium, cold tolerance, advanced backcross QTL(AB-QTL)