多粘类芽孢杆菌CP7对荔枝霜疫霉菌的抗菌活性及其作用机制

园艺学报 ›› 2010, Vol. 37 ›› Issue (7): 1047-1056.

多粘类芽孢杆菌CP7对荔枝霜疫霉菌的抗菌活性及其作用机制

陈海英1,2，林健荣1,*，廖富蘋1，林碧敏3

（1华南农业大学动物科学学院，广州 510642；2温州市农业科学研究院，浙江温州 325006；3华南农业大学公共基础教育中心，广州 510642）

收稿日期:2010-02-09 修回日期:2010-06-30 出版日期:2010-07-25 发布日期:2010-07-25
通讯作者: 林健荣

Antibacterial Activity and Mechanism of Paenibacillus polymyxa CP7 Against Peronophythora litchi

CHEN Hai-ying 1,2，LIN Jian-rong 1,*，LIAO Fu-ping 1，and LIN Bi-min 3

（1College of Animal Science，South China Agricultural University，Guangzhou 510642，China；2 Wenzhou Research Institute of Agriculture Science，Wenzhou， Zhejiang 325006，China；3 Public Elementary Education Center，South China Agricultural University，Guangzhou 510642，China）

Received:2010-02-09 Revised:2010-06-30 Online:2010-07-25 Published:2010-07-25
Contact: LIN Jian-rong

摘要/Abstract

摘要： 多粘类芽孢杆菌Paenibacillus polymyxa CP7菌株分泌的抗菌物质对荔枝霜疫霉菌等真菌具有很强的抑杀作用。用光学显微镜、扫描电镜和透射电镜观察发现，荔枝霜疫霉菌菌丝体经CP7菌株的抗菌物质处理后, 菌丝发生不规则扭曲、萎蔫、干瘪，菌丝端部膨大等形态变化；经处理的菌体细胞组织结构受损，细胞膜结构模糊不清，看不到完整的外膜结构，细胞器消溶。处理后的荔枝霜疫霉菌孢子囊出现凹陷、干瘪、畸形；孢子囊外壁严重受损，原来厚实的外壁只剩一薄层，孢子囊内原有的微细结构消失，结构模糊不清，细胞器溶解，孢囊内物质大量外泄。抗菌物质还引起菌体细胞内磷的外泄，并对细胞呼吸速率和NADH氧化酶活力有抑制作用。

关键词: 荔枝, 多粘类芽孢杆菌, 荔枝霜疫霉菌, 抗菌活性

Abstract: Paenibacillus polymyxa CP7 antibacterial substance has a significant inhibitory action on fungus, such as Peronophythora litchi. The morphology of mycelium and sporangium of Peronophythora litchi were observed under the optic microscope, the scanning electronic microscope and transmission electronic microscope. The mycelium of Peronophythora litchi treated by CP7 antibacterial substance show morphological aberrance such as irregular distorted, wizened and swelled of mycelium. It also show the expanded end of the mycelium. The cell tissue structure of mycelium is damaged that showing the incompleted cell wall and the cell membrane, the blurred cell membrane structure, incompleted external membrane structure, and the dissolved partial cell organs. The sporangium of Peronophythora litchi treated by CP7 antibacterial substance become hollow, withered and abnormal. The wall of sporangium is injuried seriously that original massiness external cell wall only remain a thin layer, original fine structure of the　sporangium is disappeared. It causes the blurred cell structure, dissolved cell organs and leakage of substances of the sporangium. In addition, the antibacterial substance also causes bacterial cells releasing of phosphorus, and inhibit cell respiration rate and NADH oxidase activity.

Key words: litchi, Paenibacillus polymyxa, Peronophythora litchi, antifungal activity

中图分类号:

S 667.1

陈海英;林健荣;廖富蘋;林碧敏. 多粘类芽孢杆菌CP7对荔枝霜疫霉菌的抗菌活性及其作用机制[J]. 园艺学报, 2010, 37(7): 1047-1056.

CHEN Hai-ying;LIN Jian-rong;LIAO Fu-ping;and LIN Bi-min . Antibacterial Activity and Mechanism of Paenibacillus polymyxa CP7 Against Peronophythora litchi[J]. ACTA HORTICULTURAE SINICA, 2010, 37(7): 1047-1056.

[1]	张树飞, 乔方, 胡桂兵, 赵杰堂, 吴河坤, 林华杰, 刘成明, 傅嘉欣, 冯奇瑞, 秦永华. 晚熟荔枝新品种‘红脆糯’[J]. 园艺学报, 2022, 49(S2): 73-74.
[2]	叶向阳, 吴晓方, 李花, 林锦何, 马锞, 叶翰江, 李建国, 叶兆雄, 王泽槐. 晚熟荔枝新品种‘桂爽’[J]. 园艺学报, 2021, 48(S2): 2817-2818.
[3]	秦雅琪，胡桂兵，赵杰堂*. 发根农杆菌介导的荔枝LcMYB1转化烟草叶片的研究[J]. 园艺学报, 2020, 47(4): 635-642.
[4]	申顺善1，张涛2，王娟1，刘东平1，张珊珊1，孙治强2，朴凤植2,*. 多粘类芽孢杆菌HK18-8对辣椒炭疽病菌的抑制作用及其定殖能力[J]. 园艺学报, 2019, 46(3): 499-507.
[5]	马锞1，赖旭辉1，胡锐清1，罗诗1，李建国2,*. 荔枝新品种‘冰荔’[J]. 园艺学报, 2019, 46(3): 605-606.
[6]	高兆银1，胡美姣1，赵超2，张正科3，李敏1，李焕苓1，王果1，孙进华1，文婕2，王家保1,*. 荔枝能荷水平及能量相关基因表达与采后褐变关系的研究[J]. 园艺学报, 2018, 45(1): 51-60.
[7]	魏永赞1，胡福初2，郑雪文1，石胜友1，董晨1，王弋1，李伟才1,*. 光照对荔枝果实着色和花色素苷生物合成影响的分子机制研究[J]. 园艺学报, 2017, 44(7): 1363-1370.
[8]	徐宁1，曾世江2，朱建华1,*，潘俭2，黄济良2，秦献泉1，彭宏祥1，梁宗活2，蒙春玲2，陈铁2，杨杰2，谭识良2，劳绍贞2，谭征2，李冬波. 荔枝新品种‘桂荔2号’[J]. 园艺学报, 2017, 44(5): 1011-1012.
[9]	马锞，谷超，刘远昌，胡锐清，罗诗，刘纪堂，王泽槐，李建国. 荔枝新品种‘唐夏红’[J]. 园艺学报, 2016, 43(8): 1621-1622.
[10]	刘锴栋, 黄素娜, 姜艳, 黎海利, 袁长春, 刘金祥, 陈燕. 番荔枝开花调控转录因子基因AsLEAFY的克隆与表达分析[J]. 园艺学报, 2015, 42(8): 1467-1476.
[11]	王树军, 冯超, 王凌云, 李焕苓, 刘保华, 王家保. 荔枝类甜蛋白基因的克隆与表达分析[J]. 园艺学报, 2015, 42(7): 1385-1392.
[12]	刘锴栋，袁长春*，黎海利，陈燕，刘金祥. 番荔枝节律钟输出基因AsGI的克隆、亚细胞定位与表达分析[J]. 园艺学报, 2015, 42(12): 2395-2404.
[13]	邢诒旺 1,2，吴开茂3，符文英2,*，吕和金3，邢诒师3. 无核荔枝新品种‘南岛无核’[J]. 园艺学报, 2015, 42(11): 2319-2320.
[14]	刘成明, 胡桂兵, 黄穗生, 黄旭明, 胡又厘, 傅嘉欣, 周佳, 秦永华, 苏灿湖, 苏有权, 倪耀源, 张永福, 潘丽佳. 晚熟优质荔枝新品种‘庙种糯’[J]. 园艺学报, 2014, 41(3): 595-596.
[15]	王凌云1,2，孙进华1，刘保华1，王家保1,. 荔枝水孔蛋白基因LcPIP*的克隆与组织特异性表达研究[J]. 园艺学报, 2013, 40(8): 1456-1464.