‘蛇龙珠’等酿酒葡萄品种的DNA分析

园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 604-608.

‘蛇龙珠’等酿酒葡萄品种的DNA分析

姚玉新1;左方梅1;翟　衡1;刘来馨2

(¹ 山东农业大学园艺科学与工程学院, 泰安271018; ² 烟台中粮长城葡萄酒公司, 蓬莱265608)

收稿日期:2004-12-27 修回日期:2005-05-23 出版日期:2005-08-25 发布日期:2005-08-25

The RAPD and SSR Analysis of ‘Cabernet Shelongzhu’and Other Wine Grape Cultivars

Yao Yuxin1;Zuo Fangmei1;Zhai Heng1;Liu Laixin2

(¹College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China;² Yantai COFCO W inery, Penglai 265608, China)

Received:2004-12-27 Revised:2005-05-23 Online:2005-08-25 Published:2005-08-25

摘要/Abstract

摘要： 为了进一步明确蛇龙珠品种的来源和亲缘关系, 利用36个RAPD引物对14个酿酒葡萄品种基因组DNA扩增, 通过聚类分析将其划分为4个类群, 其中赤霞珠、品丽珠、美乐和蛇龙珠同属于一个类群。利用SSR技术对该类群(含3个不同地区来源的蛇龙珠) 进一步分析结果表明: 蛇龙珠、赤霞珠、品丽珠和美乐是4个独立的品种, 遗传距离最大的是品丽珠和赤霞珠, 为3.46, 遗传距离最近的是美乐与品丽珠, 遗传距离为2.40。就蛇龙珠不同类型而言, 3个不同地区来源的蛇龙珠遗传上存在差异(欧氏遗传距离在1.41～1.73之间) , 但明显小于与其他品种的差异。与其他品种相比, 3个地区的蛇龙珠都与品丽珠遗传距离最近(2.45～2.64) , 都与赤霞珠最远(2.83～3.32) 。

关键词: 葡萄, RAPD, SSR, 聚类分析, 起源

Abstract: ‘Cabernet Shelongzhu’ is an important grape cultivar in China used for wine. However, its genetic origin is not clear yet. In this study, depending on the RAPD (Random Amp lification Polymorphism DNA) analysis with 36 primers, 14 wine grape cultivars were classified into four groups by UPGMA ( unweighted pair group method using arithmetic average) cluster analysis. Four cultivars of them, i. e. Cabernet Sauvignon, Cabernet Shelongzhu, Cabernet Franc and Merlot, belonged to the same group. The SSR ( Simple Sequence Repeat) analysis further indicated that they were four independent cultivars. Also, the results from SSR analysis showed that three phenotypes of‘Cabernet Shelongzhu’ sampled from different regions were genetically distinct with Euclidean distances ranging from 1.41 to 1.73, which were much smaller than those among distinct cultivars. Comparingwith other cultivars, the phenotype of Cabernet Shelongzhu from Beigou at Penglai was the closest to Cabernet Franc with the Euclidean distance of 2.45, and followed by Changli and
Yantai with the Euclidean distances of 2.56 and 2.64, respectively. The historical origin of the cultivar ‘Cabernet Shelongzhu’was investigated and discussed.

Key words: Grape, RAPD, SSR, Cluster analysis, Historical origin

中图分类号:

S 663.1

姚玉新;左方梅;翟　衡;刘来馨. ‘蛇龙珠’等酿酒葡萄品种的DNA分析[J]. 园艺学报, 2005, 32(4): 604-608.

Yao Yuxin;Zuo Fangmei;Zhai Heng;Liu Laixin. The RAPD and SSR Analysis of ‘Cabernet Shelongzhu’and Other Wine Grape Cultivars[J]. ACTA HORTICULTURAE SINICA, 2005, 32(4): 604-608.

[1]	王晓晨, 聂子页, 刘先菊, 段伟, 范培格, 梁振昌, . 脱落酸对‘京香玉’葡萄果实单萜物质合成的影响[J]. 园艺学报, 2023, 50(2): 237-249.
[2]	王瑞, 洪文娟, 罗华, 赵丽娜, 陈颖, 王君, . 石榴品种SSR指纹图谱构建及杂种父本鉴定[J]. 园艺学报, 2023, 50(2): 265-278.
[3]	刘艺平, 倪梦辉, 吴芳芳, 刘红利, 贺丹, 孔德政. 荷花花器官性状与SSR标记的关联分析[J]. 园艺学报, 2023, 50(1): 103-115.
[4]	王宝亮, 刘凤之, 冀晓昊, 王孝娣, 史祥宾, 张艺灿, 李鹏, 王海波. 早熟鲜食葡萄新品种‘华葡早玉’[J]. 园艺学报, 2022, 49(S2): 33-34.
[5]	王宝亮, 王海波, 冀晓昊, 王孝娣, 史祥宾, 王志强, 王小龙, 刘凤之. 中熟鲜食葡萄新品种‘华葡黄玉’[J]. 园艺学报, 2022, 49(S2): 35-36.
[6]	牛早柱, 赵艳卓, 陈展, 宣立锋, 牛帅科, 魏建国, 褚凤杰, 杨丽丽. 晚熟无核葡萄新品种‘紫龙珠’[J]. 园艺学报, 2022, 49(S2): 37-38.
[7]	师校欣, 杜国强, 杨丽丽, 乔月莲, 黄成立, 王素月, 赵跃欣, 魏晓慧, 王莉, 齐向丽. 晚熟无核葡萄新品种‘红峰无核’[J]. 园艺学报, 2022, 49(S2): 39-40.
[8]	吴月燕, 陈天池, 王立如, 韩善琪, 付涛. 鲜食葡萄新品种‘甬早红’[J]. 园艺学报, 2022, 49(S2): 41-42.
[9]	王晓玥, 闫爱玲, 张国军, 王慧玲, 任建成, 刘振华, 孙磊, 徐海英, . 葡萄新品种‘瑞都晚红’[J]. 园艺学报, 2022, 49(S1): 29-30.
[10]	杨秀伟, 苏江硕, 张飞, 管志勇, 房伟民, 陈发棣. 切花小菊花序开放角度的量化评价与变异分析[J]. 园艺学报, 2022, 49(8): 1723-1734.
[11]	王勇健, 孔俊花, 范培格, 梁振昌, 金秀良, 刘布春, 代占武. 葡萄表型组高通量获取及分析方法研究进展[J]. 园艺学报, 2022, 49(8): 1815-1832.
[12]	魏晓羽, 王跃进. 中国野生葡萄果皮解剖结构与白粉病抗性的相关性研究[J]. 园艺学报, 2022, 49(6): 1200-1212.
[13]	邹学校, 朱凡. 辣椒的起源、进化与栽培历史[J]. 园艺学报, 2022, 49(6): 1371-1381.
[14]	刘众杰, 郑婷, 赵方贵, 傅伟红, 诸葛雅贤, 张志昌, 房经贵. 葡萄砧木对渗透胁迫的抗性差异及生理响应机理[J]. 园艺学报, 2022, 49(5): 984-994.
[15]	梁晨, 孙如意, 向锐, 孙艺萌, 师校欣, 杜国强, 王莉. 葡萄生长调控因子GRF家族基因的鉴定及表达分析[J]. 园艺学报, 2022, 49(5): 995-1007.