低温胁迫下长豇豆幼苗可溶性蛋白质和细胞保护酶活性的变化

园艺学报 ›› 2005, Vol. 32 ›› Issue (5): 911-913.

低温胁迫下长豇豆幼苗可溶性蛋白质和细胞保护酶活性的变化

陈禅友1, 2;汪汇东2;丁　毅1

(¹ 武汉大学教育部植物发育生物学重点实验室, 武汉大学生命科学学院, 武汉430072; ² 江汉大学医学与生命科学学院, 武汉430056)

收稿日期:2004-12-11 修回日期:2005-06-28 出版日期:2005-10-25 发布日期:2005-10-25

Changes of Soluble Protein Content and Activities of Cell Protective Enzymesunder Chilling Stress in Asparagus Bean Seedlings

Chen Chanyou1, 2;Wang Huidong2;Ding Yi1

(¹Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072,China; ² School of Medicine and Life Sciences, Jianghan University, Wuhan 430056, China)

Received:2004-12-11 Revised:2005-06-28 Online:2005-10-25 Published:2005-10-25

摘要/Abstract

摘要： 对12个长豇豆品种幼苗进行10℃低温胁迫处理, 室温(20 ±3) ℃作对照, 5 d后测定叶片可溶性蛋白质含量和4种细胞保护酶活性。结果表明: 低温胁迫下, 6个早熟品种可溶性蛋白质含量较相应对照显著升高2.3%～8.1% , 3个晚熟品种显著降低; 8个品种SOD活性显著升高27.82% ～128.52%; 4个早熟品种APX活性显著升高10.53%～39.36% , 3个中晚熟品种略有降低。这3项指标能反映豇豆品种耐寒性, 耐寒性品种3项指标升高, 以SOD活性变化最剧烈。CAT和POD活性变化不适合作为长豇豆耐寒性鉴定指标。

关键词: 豇豆, 长豇豆, 低温胁迫, 可溶性蛋白质, 细胞保护酶活性

Abstract: The soluble protein content ( SPC) and activities of 4 cell protective enzymes ( SOD, CAT,POD and APX) of 12 asparagus bean cultivars under 10℃chilling stress for 5 days with the control of (20 ±3) ℃ were tested. The results showed that the SPC of leaves of seedlings raised significantly by 2.3% - 8.1%
in 6 early maturing cultivars under chilling stress compared with that of relative control, whereas 3 late maturing cultivars inversed, so did the activities of SOD and APX by 27.82% - 128.52% and 10.53% - 39.36% in 8 cultivars and 4 early maturing cultivars respectively. The three indices were sensitive to chilling stress and were suitable to identify the cold resistance of asparagus bean genotypes. The cold resistant cultivars exerted their SPC and activities of SOD and APX to acclimate chilling stress. The changes of activities of CAT and POD could hardly reflect cold resistance of asparagus bean cultivars.

Key words: V igna, Asparagus bean, Chilling stress, Soluble protein, Activities of cell protective enzyme

中图分类号:

S 643.3

陈禅友;;汪汇东;丁　毅. 低温胁迫下长豇豆幼苗可溶性蛋白质和细胞保护酶活性的变化[J]. 园艺学报, 2005, 32(5): 911-913.

Chen Chanyou;;Wang Huidong;Ding Yi. Changes of Soluble Protein Content and Activities of Cell Protective Enzymesunder Chilling Stress in Asparagus Bean Seedlings[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 911-913.

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