关键词: 番茄, 酸性转化酶, RT-PCR, 序列分析, 基因表达

Abstract: According to the published gene sequences of cell wall2binding and soluble acid invertase of tomato, two pairs of primers were designed respectively. The cDNA fragments were obtained by reverse transcriptional polymersae chain reaction (RT-PCR) using the whole RNA of different genotype tomatos, ‘Liaoyuan Duoli’, ‘Momotaro’and wild type (Lycopersicon chmielewskii) , as the templates. The fragments were cloned into pMD18-T vector and then sequenced. The sequences showed highly homology ( to 99.5% )to the published sequences of soluble and insoluble invertases, respectively. Northern blotting was performed to analyze the exp ression pattern of acid invertase in different development stages of tomato fruit of‘Liaoyuan Duoli’. The result showed that soluble acid invertase was highly expressed in pectinic placenta of mature tomato fruit, but no expression was detected in other parts of fruits.

Key words: Tomato, Acid invertase, RT-PCR, Sequence analysis, Gene expression