切花月季失水胁迫耐性差异与内肽酶活性的关联

园艺学报 ›› 2005, Vol. 32 ›› Issue (5): 854-860.

切花月季失水胁迫耐性差异与内肽酶活性的关联

赵喜亭;丛日晨;单宁伟;高俊平

(中国农业大学观赏园艺与园林系, 北京100094)

收稿日期:2004-10-25 修回日期:2005-01-05 出版日期:2005-10-25 发布日期:2005-10-25

Relationship between Endopeptidase Activity in Petals and Tolerance to Water Deficit Stress of Two Cut Rose Cultivars

Zhao Xiting;Cong Richen;Shan Ningwei;Gao Junping

(Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China)

Received:2004-10-25 Revised:2005-01-05 Online:2005-10-25 Published:2005-10-25

摘要/Abstract

摘要： 选用切花月季耐失水胁迫品种‘Samantha’和中度耐失水胁迫品种‘Belami’, 采用带枝花和离枝花两种形式, 在22～25℃、RH 30%～50%、80μE·m^{- 2} ·s^{- 1}荧光灯照光条件下进行了0～60 h的失水胁迫处理。结果显示: ①失水胁迫中花枝弯颈率的增加, ‘Samantha’快于‘Belami’, 同一品种带枝花快于离枝花, 但复水后恢复率的降低前者慢于后者, 同一品种带枝花慢于离枝花。胁迫过程中, 带枝花、离枝花都出现了恢复率快速降低的拐点, 前者晚于后者。对应于花枝复水恢复率快速降低的拐点, 水势值同一品种的带枝花、离枝花基本相同。②两品种花瓣中游离氨基酸含量均是带枝花、离枝花快速上升的转折点与其相应的复水率迅速降低拐点相吻合。③胁迫诱导的花瓣内肽酶活性上升, ‘Samantha’明显晚于‘Belami’, 同一品种带枝花早于离枝花。两品种带枝花和离枝花花瓣内肽酶活性诱导上升的转折点与其相应的复水恢复率迅速降低拐点相一致。④胁迫诱导引起的花瓣中金属蛋白酶和丝氨酸蛋白酶活性的变化均是‘Samantha’显著低于‘Belami’。由此推测, 切花月季品种间失水胁迫耐性差异可能与胁迫对花瓣中金属蛋白酶和丝氨酸蛋白酶活性诱导的差异有关。

关键词: 月季, 切花, 失水胁迫, 耐性, 内肽酶

Abstract: This work was aimed to investigate to relations between tolerance to water deficit stress (WDS) and the activity and type of endopep tidase in different cultivars of Cut roses (Rosa hybrid). Two cultivars, ‘Samantha’with the strong tolerance to WDS, and ‘Belami’with the medium one, were used as
materials, and WDS treatment was carried out for 0 - 60 h under the conditions of 22 - 25℃, RH 30% - 60% , and 80μE·m^{- 2} · s^{- 1}. To make different p rocess ofWDS, we treated the two cultivars separately with two manners, namely flowers with and without branches in the same condition of WDS above. The results showed that, ① the decrease of flower recovery percentage was much slower in ‘Samantha’ than in‘Belami’, and was slower significantly in the flowerswith branches than without branches in a cultivar, while the opposite tendency was obtained on the increase of flower bent neck percentage during WDS. A turning point, in which recovery rate of flowers was going to be decreasing rapidly, was observed in both flowers with and without branches, and the occurrence of the turning pointwas later in ‘Samantha’ than in ‘Belami’. However, the value of water potential was almost the same in a cultivar, corresponding to the turning point of recovery rate. ② The turning point of quick climbing of free amino acid content in petals was also observed in
the flowers of both cultivars, including the two manners, with orwithout branches separately duringWDS, and these pointswere identicalwith the turning points of their recovery rate. ③WDS2induced increase of endopeptidase activity was later in‘Samantha’than in‘Belami’, and it was earlier in the flowerswith branches than without branches in a cultivar. Interestingly, the turning points of quick increase of endopep tidase activity in2duced byWDS in both cultivarswith orwithout brancheswere consistentwith the turning points of their recovery rates. ④ Changes of both metall-oproteinase and serine proteinase activities of petals induced by WDS were significantly smaller in ‘Samantha’ than in ‘Belami’. The results above suggested that the difference of tolerance toWDS between different cultivars might be related to the different changes of metallo-proteinase and serine proteinase activitives in petals induced by WDS.

Key words: Rose, Cut flower, Tolerance, Water deficit stress, Endopep tidase

中图分类号:

S 685.12

赵喜亭;丛日晨;单宁伟;高俊平. 切花月季失水胁迫耐性差异与内肽酶活性的关联[J]. 园艺学报, 2005, 32(5): 854-860.

Zhao Xiting;Cong Richen;Shan Ningwei;Gao Junping. Relationship between Endopeptidase Activity in Petals and Tolerance to Water Deficit Stress of Two Cut Rose Cultivars[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 854-860.

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