不同倍性百合杂交后代的核型及分子标记鉴定

园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1477-1484.

不同倍性百合杂交后代的核型及分子标记鉴定

陈琼^1，2;穆鼎^1*;义鸣放²;明军¹;刘春¹

（¹中国农业科学院蔬菜花卉研究所，北京 100081；²中国农业大学园林植物与观赏园艺系，北京 100094）

收稿日期:2007-05-08 修回日期:2007-10-11 出版日期:2007-12-25 发布日期:2007-12-25
通讯作者: 穆鼎

Identification and Genetic Expressions of Lily Hybrids Obtained by Different Ploidy Cross Combinations

CHEN Qiong^1,2, MU Ding^1*, YI Ming-fang², MING Jun¹,and LIU Chun¹

（¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China; ²Department of Ornamental Horticulture, China Agricultural University, Beijing 100094, China）

Received:2007-05-08 Revised:2007-10-11 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 以百合两个杂交组合，'Brunello'×'Yellow Jiant'和'Prato'×Lilium.pumilum的亲本和杂交后代为试材，从染色体和DNA两个方面对杂交后代进行鉴定，比较杂交后代与亲本间的遗传差异和相似性。观察显示，两个杂交后代均为三倍体，由不同倍性的亲本杂交而来，且染色体随体丰富。用4对多态性高的SRAP引物对试材进行扩增。结果表明：杂交后代除了含有父母本的特征带，还具有父母本没有的谱带，且它们与母本间的遗传距离均小于其与父本间的遗传距离。核型分析和分子标记的结果一致，均证实了两个杂交后代的真实性，且两个杂交后代在遗传上均偏向母本。

关键词: 百合, 杂交, 核型分析, SRAP

Abstract: Two hybrids, 'Brunello'× 'Yellow Jiant' and 'Prato'×Lilium pumilum, were identified by karyotype analysis and SRAP techniques. Genetic differences and similarity were compared between the parents and the progenies. The results showed that the two hybrids were both triploids, and contain abundant trabant. Four pairs of SRAP primer with higher polymorphism were used and the hybrid bands and complementary bands were found in the hybrids. Pairwise genetic distances between the hybrids and their female parents are smaller than that between the progenies and their male parents. The results of SRAP were in consistent with that of karyotype analysis. The two hybrids were both true progeny of their parents, and both with a genetic tendency to their female parents.

Key words: Lily, Hybridization, Chromosome, Polyploidy, Karyotype analysis

中图分类号:

S 682.2

陈琼;穆鼎;义鸣放;明军;刘春. 不同倍性百合杂交后代的核型及分子标记鉴定[J]. 园艺学报, 2007, 34(6): 1477-1484.

CHEN Qiong;MU Ding;YI Ming-fang;MING Jun;and LIU Chun. Identification and Genetic Expressions of Lily Hybrids Obtained by Different Ploidy Cross Combinations[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1477-1484.

[1]	朱炜, 曹津津, 陈曦, 张葳, 孙榕泽, 朱绍才, 赵家庚, 崔雅琦, 王宇暄, 于晓南. 芍药新品种‘粉罗裙’‘千重粉黛’‘笑春风’和‘碧叶粉荷’[J]. 园艺学报, 2023, 50(2): 457-458.
[2]	杨植, 张川疆, 杨芯芳, 董梦怡, 王振磊, 闫芬芬, 吴翠云, 王玖瑞, 刘孟军, 林敏娟. 枣与酸枣杂交后代果实遗传倾向及混合遗传分析[J]. 园艺学报, 2023, 50(1): 36-52.
[3]	蔡鹏, 房超, 李跃建, 刘独臣, 刘小俊, 梁根云. 茄子新品种‘天骄’[J]. 园艺学报, 2023, 50(1): 229-230.
[4]	许申平, 袁秀云, 张燕, 何培, 崔波. 铁皮石斛新品种‘豫斛4号’[J]. 园艺学报, 2023, 50(1): 233-234.
[5]	邓朝军, 许奇志, 蒋际谋, 胡文舜, 郑少泉, 陈秀萍, 姜帆, 许家辉, 苏文炳, 张雅玲, 黄敬峰. 浓香型龙眼新品种‘醇香’[J]. 园艺学报, 2022, 49(S2): 75-76.
[6]	邓朝军, 陈秀萍, 许奇志, 蒋际谋, 郑少泉, 胡文舜, 姜帆, 许家辉, 苏文炳, 张雅玲, 黄敬峰. 浓香型龙眼新品种‘福香’[J]. 园艺学报, 2022, 49(S2): 77-78.
[7]	欧阳丽莹, 闫凯, 李春文, 雷应才, 张丽婷, 练华山, 杜英杰. 苦瓜新品种‘金田186’[J]. 园艺学报, 2022, 49(S2): 129-130.
[8]	余文迪, 姜贵芸, 刘娟旭, 余义勋, . 报春苣苔属新品种‘仙女棒’[J]. 园艺学报, 2022, 49(S2): 185-186.
[9]	姜贵芸, 余文迪, 刘娟旭, 余义勋, . 报春苣苔属新品种‘淡妆仙子’[J]. 园艺学报, 2022, 49(S2): 187-188.
[10]	孔令普, 欧阳雪灵, 彭火辉, 叶川, 袁淑贞, 王国行, 彭玉辅, . 报春苣苔属新品种‘初心’[J]. 园艺学报, 2022, 49(S2): 189-190.
[11]	黄扬, 邓涛, 唐文秀. 秋海棠新品种‘花脸’[J]. 园艺学报, 2022, 49(S2): 201-202.
[12]	樊金萍, 戴静琪, 江翼帆, 闫凯丽, 张金柱, 杨涛, 车代弟. 百合新品种‘冰粉皇后’[J]. 园艺学报, 2022, 49(S2): 205-206.
[13]	谢添伟, 王安石, 林明光, 陈施明, 韩松, 潘英文, 刘福秀. 苞舌兰属新品种‘克保’[J]. 园艺学报, 2022, 49(S2): 209-210.
[14]	何智宏, 何丽霞, 张延东, 杨国州, 李睿, 徐晶晶, 瞿丹, 李京璟. 牡丹新品种‘余霞散绮’[J]. 园艺学报, 2022, 49(S2): 219-220.
[15]	田晓玲, 马永鹏, . 高山杜鹃新品种‘流光溢彩’[J]. 园艺学报, 2022, 49(S2): 245-246.