蝶形花亚科8种槐树的组织培养及再生能力的基因型效应

园艺学报 ›› 2005, Vol. 32 ›› Issue (5): 844-848.

蝶形花亚科8种槐树的组织培养及再生能力的基因型效应

王关林1;刘秀梅1;方宏筠1;李　红1;李华刚2

(¹ 辽宁师范大学生命科学学学院, 大连116029; ² 宝生物工程有限公司, 大连116600)

收稿日期:2004-12-21 修回日期:2005-04-20 出版日期:2005-10-25 发布日期:2005-10-25

Plant Tissue Culture for 8 Pagoda Tree Var ieties of Papilionoideae and Effects of Genotypes on Regeneration Ability

Wang Guanlin1;Liu Xiumei1;Fang Hongjun1;Li Hong1;Li Huagang2

(¹College of Life Sciences, Liaoning Normal University, Dalian 116029, China; ²Bao Bioengenierring, Dalian 116600, China)

Received:2004-12-21 Revised:2005-04-20 Online:2005-10-25 Published:2005-10-25

摘要/Abstract

摘要： 对国槐等8种(亚种) 蝶形花亚科植物组织培养再生能力的研究结果表明: 8种槐树叶片的再生能力相差较大, 不定芽分化率由高到低排序为金叶槐( 79.03% ) 、黄金槐( 41.46% ) 、香花槐
(36.2% ) 、刺槐(34.2% ) 、黄花槐(25% ) 、国槐(22.7% ) 、四倍体刺槐(21% ) 、红花槐(18% ) ; 愈伤组织诱导率、芽分化率及试管苗的生根率排列次序与叶片芽分化率基本相同。从本试验结果可得出8种槐树基因型对组织培养再生能力具有如下调控效应及特点: 属间、种间及亚种间基因型对培养基种类及植物生长调节剂的要求, 对脱分化与再分化能力的调控, 对不同组织、器官的再生能力的调控均具有同一性;不同基因型、甚至同一基因型的不同组织、器官还具有一定的特异性。

关键词: 槐树, 组织培养, 基因型, 再生能力

Abstract: The regeneration ability for 8 pagoda tree varieties of Papilionoideae in the plant tissue culture were compared with different genotypes. The result shows that the buds differentiation rate of leaves varied much with a descending order var. japonica Jinye ( 79.03% ) , var japonica golden ( 41.46%) , Cladrastis sinensis (36.2% ) , Robinia psudoacian L. ( 34.2% ) , Cassia suffruticasa ( 25% ) , Sophora japonica L. (22.7% ) , var. robinia tetrap loid (21% ) and Robinia hispida L. (18% ) ; The descending order of induction rate, differentiation rate of cullas and rooting rate was the alide with the bud differentiation rate of leaves. The regulatory effects and traits of genotype on plant regeneration abilitywas discussed respectively by this experiment system. There were indications that the demand for basic medium and foreign hormone was similar between different genotypes of pagoda tree; the regulation of genotype on dedifferentiation and redifferentiation of pagoda trees was identical; the regulation of genotype had identity on regeneration ability of plant tissues and organs, but there still existed specificities in different tissues and organs of different genotypes as well as the same genotype.

Key words: Pagoda tree, Genotype, Tissue culture, Regeneration ability

中图分类号:

S 68

王关林;刘秀梅;方宏筠;李　红;李华刚. 蝶形花亚科8种槐树的组织培养及再生能力的基因型效应[J]. 园艺学报, 2005, 32(5): 844-848.

Wang Guanlin;Liu Xiumei;Fang Hongjun;Li Hong;Li Huagang. Plant Tissue Culture for 8 Pagoda Tree Var ieties of Papilionoideae and Effects of Genotypes on Regeneration Ability[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 844-848.

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