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园艺学报 ›› 2005, Vol. 32 ›› Issue (5): 778-782.

• 研究论文 • 上一篇    下一篇

苹果果实酚类物质含量与果实苦涩关系的研究

乜兰春;孙建设   

  1. (河北农业大学园艺学院, 保定071001)
  • 收稿日期:2004-11-02 修回日期:2005-01-13 出版日期:2005-10-25 发布日期:2005-10-25

Relationship between the Content of Phenolic Compounds and the Taste ofAstringency and Bitterness in Apple Fruit

Nie Lanchun;Sun Jianshe   

  1. (College of Horticulture, Agricultural University of Hebei, Baoding 071001, China)
  • Received:2004-11-02 Revised:2005-01-13 Online:2005-10-25 Published:2005-10-25

摘要: 以富士苹果为试材, 利用高效液相色谱(HPLC) 研究了果实酚类物质含量与果实苦涩的关系以及酚类物质在果实中的分布。结果表明, 苦涩的幼果中, 根皮素和儿茶素含量均高达成熟果实的30多倍; 表儿茶素、原花青素和绿原酸含量分别为成熟果实的10倍左右。在成熟果实中, 有涩味的果实其绿原酸、儿茶素、表儿茶素和原花青素含量显著高于风味正常的果实; 有苦味的果实其根皮素和原花青素含量显著高于风味正常的果实; 苦涩味均较强的果实其绿原酸、儿茶素、表儿茶素、根皮素和原花青素含量最高。苦痘病果病变组织中根皮素和原花青素含量分别是正常组织的15倍和3倍。绿原酸、儿茶素、表儿茶素和原花青素是引起苹果果实涩感的主要物质; 根皮素、儿茶素和原花青素是引起苦味的主要物质。成熟果实中绿原酸主要分布于种子、果心和果肉; 儿茶素、表儿茶素和槲皮素主要分布于果皮; 原花青素主要分布于果皮中, 果肉和果心中也有分布; 根皮素则主要分布于种子、果皮和果心中。

关键词: 苹果, 酚类物质

Abstract: The distribution of phenolic compounds and the relationship between the content of phenolic compounds and the taste of astringency and bitterness in apple (Malus domestica Borkh. ) fruits were studied by high-performance liquid chromatography (HPLC). The results showed that the contents of phloridzin and catechin were more than 30 times, and epicatechin, procyanidins and chlorogenic acid were about 10 times in young fruits with strongly astringency and bitterness compared to that in ripe fruitswith no taste of astringency
and bitterness. In ripe fruits with astringent taste, the contents of chlorogenic acid, catechin, epicatechin, and procyanidinswere much higher than that with no astringent taste. In fruits with bitter taste, the contents of chlorogenic acid, phloridzin, and procyanidinswere much higher than that with no bitter taste. In fruits with both astringent and bitter taste, the contents of chlorogenic acid, catechin, epicatechin, phloridzin, and p rocyanidins were the highest. The levels of phloridzin and procyanidins in the bitter pit tissue were fifteen and three times higher than those in normal tissue, respectively. These results indicated that the astringency of apple fruitswas due to the increase of chlorogenic acid, catechin, epicatechin, and procyanidins, while the bitter taste was associated with the accumulation of phloridzin, catechin, and p rocyanidins. Chlorogenic acid mainly exists in seed, core, and flesh. Catechin, epicatechin, and quercetin are mainly in the peel. Procyanidins are also mainly in the peel with small amount in the flesh and core, and phloridzin is mainly in the seed, peel, and core.

Key words: Apple, Phenolic compounds

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