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园艺学报 ›› 2010, Vol. 37 ›› Issue (6): 1009-1014.

• 研究报告 • 上一篇    下一篇

山药糖蛋白体外抗氧化活性研究

孙宇婧1,韩 涛1,*,李丽萍2,杨 蕊1   

  1. (1北京农学院食品科学系,北京102206;2北京工商大学植物资源研究开发北京市重点实验室,北京100037)
  • 收稿日期:2010-02-11 修回日期:2010-04-01 出版日期:2010-06-25 发布日期:2010-06-25
  • 通讯作者: 韩 涛

Antioxidant Activity in Vitro of Glycoprotein from Chinese Yam

SUN Yu-jing1,HAN Tao1,*,LI Li-ping2,and YANG Rui1   

  1. (1Department of Food Science,Beijing University of Agriculture,Beijing 102206,China;2Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology & Business University,Beijing 100037,China)
  • Received:2010-02-11 Revised:2010-04-01 Online:2010-06-25 Published:2010-06-25
  • Contact: HAN Tao

摘要: 采用热水浸提法提取山药糖蛋白,经DEAE-52和SephadexG-75柱层析纯化,得到两种山药糖蛋白纯品,分析其体外抗氧化活性,包括还原能力以及对H2O2、、·OH、DPPH的清除作用、对脂质体过氧化的抑制能力,并分别与抗坏血酸效果作比较。结果表明,山药糖蛋白具有较高的还原能力,对H2O2、、·OH、DPPH有一定的清除能力,对脂质体过氧化具有抑制作用。山药糖蛋白的抗氧化活性在一定浓度范围内(0 ~ 20 mg · mL-1)存在量效关系,随着山药糖蛋白浓度的增加,其还原能力和对H2O2、、· OH、DPPH清除能力增加,但对脂质体过氧化的抑制是先增加后降低。在相同浓度下,山药糖蛋白CYG-1的抗氧化能力稍弱于山药糖蛋白CYG-2。

关键词: 山药, 糖蛋白, 抗氧化活性, 体外

Abstract: After yam flesh were exposed to hot water extraction and then purified by DEAE-52 and SephadexG-75 columns,two pieces of glycoproteins in Chinese yam were obtained,i.e. CYG-1 and CYG-2. The antioxidant activities in vitro of the two glycoproteins were investigated. The results showed that the two glycoproteins had scavenging activities against hydroxyle radical(·OH),hydrogen peroxide (H2O2),superoxide anion radical()and 1,1-diphenyl-2-picryl-hydrazyl(DPPH)as well as inhibiting capacity of lipidsome peroxidation. There were positive relations between CYGs concentrations and their antioxidant activities. There were some differences on antioxidant activities between CYG-1 and CYG-2. Generally,CYG-2 exhibited better effects than CYG-1.

Key words: Chinese yam, Dioscorea opposite Thumb., glycoprotein, antioxidation activity, in vitro

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