中国兰属植物种间及品种间亲缘关系的RAPD分析

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1121-1124.

中国兰属植物种间及品种间亲缘关系的RAPD分析

孙彩云;张明永;叶秀粦;梁承邺;夏快飞

(中国科学院华南植物园, 广州510650)

收稿日期:2004-12-22 修回日期:2005-05-11 出版日期:2005-12-25 发布日期:2005-12-25

Studies on Relationship between Species, Cultivars of Cymbidium Using RAPD

Sun Caiyun;Zhang Mingyong;Ye Xiulin;Liang Chengye;Xia Kuaifei

(South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China)

Received:2004-12-22 Revised:2005-05-11 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 利用RAPD分子标记分析中国兰属(Cymbidium ) 的28个原生种和部分种的不同品种及3个杂交种共50个材料间的亲缘关系。获得250条可明显区分的DNA扩增片段, 除S143-850 bp 1条带为全部材料共有外, 其余带均为多态性位点。这些带最大的为2 100 bp, 最小的为200 bp, 以集中在1 031～300 bp的带居多。UPGMA进行聚类分析显示: RAPD的聚类树状图所呈现出的亲缘关系大体与ITS系统发育树一致。建兰亚属可能为一自然类群。大花亚属并非自然类群, 其成员之一文山红柱兰(C. Wenshanense Y.S. Wu et. ) 偏离出去而与兰亚属聚在一起。兰亚属则为一高度异质性的类群, 分散成互不关联的3支。本结果和ITS系统发育分析表明, 有必要对经典分类的兰属属下分类, 特别是兰亚属进行修订。对3个亚属中各组的划分基本与传统分类的一致。春兰品种间的多样性高于其它测试品种。

关键词: 兰属, 兰科, RAPD

Abstract: Cymbidium is a genus with very important values of horticulture. In the present study genetic closeness of various species of Cymbidium was determined using RAPD. A total of 250 amplified bands was gotten from all PCR reactions by 12 selected primers, and their band size was from 200 bp to 2 100 bp. Except of the 850 bp band amplified from S143 primer, all other amp lified bands were diversity locus, and most of the bands were located between 1 030 bp and 300 bp. The UPGMA dendrogram showed some congruence with the previous ITS phylogeny, which revealed the paraphyly of subgen. Cyperorchis, and high polyphyly of subgen. Cymbidium. The subgroups within each subgenus revealed by the RAPD were, however, correspond to the traditional taxonomy based on gross morphology. This study also shows higher genetic diversity of C. goeringii, being cultived for long history, than other Cymbidium species.

Key words: Cymbidium, Orchidaceae, RAPD

中图分类号:

S 682.31

孙彩云;张明永;叶秀粦;梁承邺;夏快飞. 中国兰属植物种间及品种间亲缘关系的RAPD分析[J]. 园艺学报, 2005, 32(6): 1121-1124.

Sun Caiyun;Zhang Mingyong;Ye Xiulin;Liang Chengye;Xia Kuaifei. Studies on Relationship between Species, Cultivars of Cymbidium Using RAPD[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1121-1124.

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