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园艺学报 ›› 2008, Vol. 35 ›› Issue (3): 329-336.

• 果树 • 上一篇    下一篇

甜樱桃品种SSR指纹检索系统的开发及遗传多样性分析

张琪静1, 2 , 张新忠3 , 代红艳1 , 谷大军2 , 闫桂军4 , 李春敏3 , 张志宏1*   

  1. (1 沈阳农业大学园艺学院, 沈阳110161; 2 辽宁省果树科学研究所, 辽宁熊岳115214; 3 河北省农林科学院昌黎果树研究所, 河北昌黎066600; 4 西澳大学, 珀斯6009, 澳大利亚)
  • 收稿日期:2007-09-01 修回日期:2008-01-12 出版日期:2008-03-25 发布日期:2008-03-25
  • 通讯作者: 张志宏

Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars

ZHANG Qi-jing1, 2 , ZHANG Xin-zhong3 , DA IHong-yan1 , GU Da-jun2 , YAN Gui-jun4 , L I Chun-min3 , and ZHANG Zhi-hong1*   

  1. (1College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; 2Liaoning Institute of Pomology, Xiongyue, Liaoning 115214, China; 3Changli Fruit Research Institute, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China; 4 The University of Western Australia, Perth 6009, Australia)
  • Received:2007-09-01 Revised:2008-01-12 Online:2008-03-25 Published:2008-03-25
  • Contact: ZHANG Zhi-hong

摘要: 用SSR技术开发了甜樱桃( Prunus avium ) 指纹检索系统并进行遗传多样性分析。18对樱
桃、桃和杏的引物在19份甜樱桃及2份草原樱桃( P. fruticosa) 品种中共扩增出83个等位位点, 每个 SSR位点的等位位点数2 ~8 个, 平均416 个, 多态性信息量( PIC) 变化范围为0.38 ~0.80, 平均为 0.64。7个甜樱桃品种具有特殊位点或特殊带型。利用UDP98-414、UDP98-406、UDP96-001及PMS40等4 对引物开发的指纹检索系统, 可以区分18个甜樱桃品种。根据遗传距离进行聚类分析, 19个甜樱桃品种 分成2组, 甜樱桃品种间的聚类结果基本反映了供试材料之间的亲缘关系。

关键词: 甜樱桃, SSR, 遗传多样性, 品种鉴定, 指纹检索系统

Abstract:

SSR technique was used to develop fingerprinting key and study the genetic diversity on sweet cherry. Eighteen pairs of primers cloned from cherry, peach and apricot amplified a total of 83 alleles among the 19 cultivars of sweet cherry ( Prunus avium ) and 2 cultivars of ground cherry ( P. fruticosa). The number of alleles amplified per primer pair ranged from 2 to 8, with a mean of 416. The polymorphic information content values ( PIC) ranged from 0.38 to 0.80, with a mean of 0.64. Seven sweet cherry cultivars showed unique bands or banding patterns. A fingerp rinting key developed by 4 pairs of p rimers: UDP98-414, UDP98-406, UDP96-001 and PMS40 could discriminate 18 sweet cherry cultivars. UPGMA cluster analysis of genetic distance separated the 19 cultivars of sweet cherry into two group s. The phylogeny of sweet cherry cultivars revealed the closed relationships among cultivars tested.

Key words: sweet cherry, SSR, genetic diversity, cultivar identification, fingerprinting key

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