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园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1531-1534.

• 研究简报 • 上一篇    下一篇

梨矮化基因pcDw的一个SCAR标记

贾彦利, 王彩虹*,田义轲, 戴洪义,王 亮   

  1. (青岛农业大学园艺学院,山东青岛 266109)
  • 收稿日期:2007-04-09 修回日期:2007-09-07 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 王彩虹

A SCAR Marker Linked to pcDw Gene in Pear

JIA Yan-li, WANG Cai-hong*, TIAN Yi-ke, DAI Hong-yi, and WANG Liang   

  1. (College of Horticulture, Qingdao Agricultural University, Qingdao,Shandong 266109, China)
  • Received:2007-04-09 Revised:2007-09-07 Online:2007-12-25 Published:2007-12-25

摘要: 以'矮化梨'(Pyrus communis L.)与 '茌梨'(Pyrus bretschneideri Rehd.)的杂交后代共111个单株为试材,采用分离群体分组分析法(Bulked Segregate Analysis, BSA),通过对412个随机引物的筛选,获得了一个与控制梨树矮化性状基因pcDw连锁距离为8.3 cM、长度为940 bp的RAPD标记S1172-940, 并将其转换成了SCAR (Sequence Characterized Amplified Region) 标记,即SCAR-940。这一研究结果,为该矮化性状的标记辅助选择提供了有效工具。

关键词: 梨, 矮化, RAPD, SCAR

Abstract: To identify molecular markers linked to pcDw gene determining the dwarf character of pear tree, a population of 111 individuals from the cross of 'Aihuali'(Pyrus communis L.)×'Chili'(Pyrus bretschneideri Rehd.) was tested using 412 random primers by the way of bulked segregate analysis(BSA). A RAPD marker, S1172-940, linked to pcDw gene at a distance of 8.3 cM, was detected. This RAPD marker was converted into a SCAR (Sequence Characterized Amplified Region) marker, SCAR-940, which would provide an effective tool for marker assisted selection for the dwarf trait.

Key words: Pear, Dwarf, RAPD, SCAR

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