关键词: 大白菜, 游离小孢子培养, 胚状体, 植株再生率

Abstract: During the microspore embryogenesis of Chinese Cabbage, the embryo development was not synchronism and cotyledonary embryos was the highest ( 80% ). The better embryos develop, the yield of higher plantlet regeneration rate is. And the regenerated plant rate of cotyledonary embryos and germinated embryos were 20% and 90.5% respectively. Dry culture medium was favor of regenerating plantlet, optimum agar content was 1.2%. Culture medium supplemented with activated charcoal 200 mg·L ^{- 1} could evidently
raise regenerated plant rate, which was as 2.225 times as control (without activated charcoal). Transferring mature cotyledonary embryos to the regenerating culture medium in time was essential to increasing plantlet regeneration rate.

Key words: Chinese cabbage, Isolated-microspore culture, Embryo, Plantlet regeneration