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园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1088-1091.

• 研究报告 • 上一篇    下一篇

雷州半岛菠萝蜜种质遗传多样性的RAPD分析

叶春海;李映志;丰锋   

  1. (广东海洋大学农学院, 湛江524088)
  • 收稿日期:2005-04-19 修回日期:2005-08-04 出版日期:2005-12-25 发布日期:2005-12-25

Analysis of Genetic Diversity of Jackfruit Germplasm Grown in Leizhou Peninsula, China, Using RAPD Marking Method

Ye Chunhai;Li Yingzhi;Feng Feng   

  1. (College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China)
  • Received:2005-04-19 Revised:2005-08-04 Online:2005-12-25 Published:2005-12-25

摘要: 用RAPD标记方法对我国雷州半岛的65份菠萝蜜(Artocarpus heterophyllus Lam. ) 实生种质资源DNA的遗传多样性进行了检测, 16个引物共检测到78条带, 其中69条具多态性(占88.4% ) 。聚类分析表明, 65份菠萝蜜材料在遗传距离0.26处可分为3大类。供试种质虽具有丰富的形态性状变异, 但在DNA水平上平均相似性系数为0.7341。干、湿胞类型及引自马来西亚的种质均不能独立聚类。对菠萝蜜品种的干、湿胞分类法及菠萝蜜品种的引种区域进行了讨论。

关键词: 菠萝蜜, 遗传多样性, 种质资源, RAPD

Abstract: RAPD ( random amplified polymorphic DNA) variation was assessed in 65 accessions of jackfruit (Artocarpus heterophyllus Lam. ) from Leizhou peninsula, southern China. Among 78 bands detected by 16 RAPD p rimers, 69 were polymorphic. The cluster analysis showed that three group s could be clustered
when genetic distance coefficient was given as 0.26. In contrast to high diversity among morphology characters, high average genetic similarity coefficient was observed (0.7431) on RAPD markers. Soft type and firm type could not form an independent cluster, nor the germplasm introduced from Malaysian species. The classification methods and the introduction strategy of jackfruit germplasm were discussed.

Key words: Jackfruit, Genetic diversity, Germplasm resources, RAPD

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