关键词: 梨, 组织培养, 植株再生

Abstract: Leaves excised from one-year old shoots of‘Xueqing’pear ( Pyrus sp. ) were used asmaterials to induce adventitious shoots on MS medium supplied with different combinations of p lant growth regulators, 8 g·L^-1 agar and 30 g·L^-1 sucrose, pH = 5.8 in a growth chamber set at a 14 h photoperiod, a 2 000
lx photosynthetic density and a (25 ±1) ℃ temperature. 100% of the leaf explants dedifferentiated onMS + 2,4-D 2 mg·L ^{- 1} + 6-BA 0.5 mg·L^-1 and calli increased 12.05 times in a 30-day subculture period. All the calli could regenerate adventitious shoots after transferred on MS + 6-BA 5 mg·L^-1 + IAA 0.1 mg·L ^{- 1} and the shoots proliferated about 7 times in every subculture during one to six subcultures on MS + 6-BA 2 mg·L ^{- 1} + IAA 0.1 mg·L ^{- 1} +CH 100 mg·L ^{- 1}. 67.50% of shoots rooted on 1 /2MS + IBA 2 mg·L ^{- 1} + 6-BA 0.5 mg·L ^{- 1} +AC 500 mg·L ^{- 1}. Of 68 plantlets, 46 plantlets (67.65% ) were acclimatized after transplanting to matrix pearlite for 30 days.

Key words: Pear, Tissue culture, Plant regeneration