非对称体细胞融合获得花椰菜与Brassica spinescens的种间杂种

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1039-1044.

非对称体细胞融合获得花椰菜与Brassica spinescens的种间杂种

姚星伟;刘　凡;云兴福;赵　泓;Ryschka U;Schumann G

(¹ 内蒙古农业大学农学院, 呼和浩特010019; ² 北京市农林科学院蔬菜研究中心, 北京100089; ³德国联邦作物育种中心园艺作物研究所, 奎德林堡 D206484)

收稿日期:2005-03-04 修回日期:2005-04-13 出版日期:2005-12-25 发布日期:2005-12-25

Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion

Yao Xingwei;Liu Fan;Yun Xingfu;Zhao Hong;Ryschka U;Schumann G

(¹Agricultural College, Inner Mongolia Agricultural University, Huhhot 010019, China; ²Beijing Vegetable Research Center, Beijing 100089, China; ³ Institute of Horticultural Crops of Federal Center forB reeding Research on Cultivated Plants, Neuer Weg 22/23, Quedlinburg D206484, Germany)

Received:2005-03-04 Revised:2005-04-13 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 拟利用非对称原生质体融合技术向芸薹属花椰菜中转移野生抗逆性状。供体Brassica spinescens具有光合效率高, 抗白锈病、蚜虫、黑斑病, 耐盐等优良特性。用经UV处理的供体叶肉原生质体与花椰菜下胚轴原生质体通过聚乙二醇( PEG) 融合, 培养后获得379株再生植株。对其中的120株进行过氧化物同工酶分析和RAPD分子标记检测, 证明有23株为杂种植株。流式细胞仪倍性分析表明, 杂种植株核DNA含量全部高于供、受体亲本核DNA含量总和, 植株倍性在四至八倍之间。还对供体不同UV射线处理的剂量进行了初步探索, 结果表明0.0750 J·cm ^{- 2}为最大辐射剂量。

关键词: 芸薹属, 种间杂种, 非对称体细胞融合, 原生质体培养, 杂种鉴定

Abstract: Asymmetric protoplast fusion between Brassica oleracea L. var. botrytis and B. spinescens was taken for transferring of wild resistant traits into B. oleracea. B. spinescens possesses desirable characteristics such as high photosynthetic efficiency, resistance to the white rust ( caused by Albugo candida) , aphid
and alternaria blights ( caused by Alternaria brassicae) and tolerance to salt. PEG ( polyethyleneglycol) induced asymmetric fusions were performed between mesophyll p rotop lasts of B. spinescens irradiated with UV and hypocotyl protoplasts of B. oleracea L. var. botrytis, and 379 regenerated plants were obtained. Peroxidase isozyme ( POX) analysis was taken in 120 plants, and 23 of them showed hybrid characters that were confirmed by the RAPD marker detection. The relative nuclearDNA content of the hybridsmeasured by flow
cytometry ( FCM) showed that all of the 23 analyzed plants had a DNA content from tetraploidy to octoploidy. Influence of UV doses had been tested, and results showed that the maximum UV dose for B. spinescens was 0.0750 J·cm^{- 2}.

Key words: Brassica, Interspecific hybrid, Asymmetric somatic fusion, Protoplast culture, Hybrid identification

中图分类号:

S 635.3

姚星伟;刘　凡;云兴福;赵　泓;Ryschka U;Schumann G. 非对称体细胞融合获得花椰菜与Brassica spinescens的种间杂种[J]. 园艺学报, 2005, 32(6): 1039-1044.

Yao Xingwei;Liu Fan;Yun Xingfu;Zhao Hong;Ryschka U;Schumann G. Interspecific Hybridization between Brassica oleracea L. var. botrytis andBrassica spinescens via Asymmetric Somatic Protoplast Fusion[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1039-1044.

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