高温胁迫下铁炮百合幼苗形态及生理反应的初步研究

园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 145-147.

高温胁迫下铁炮百合幼苗形态及生理反应的初步研究

周斯建;义鸣放;穆　鼎

(¹ 中国农业大学观赏园艺与园林系, 北京100094; ² 中国农业科学院蔬菜花卉研究所, 北京100081)

收稿日期:2004-06-24 修回日期:2004-11-17 出版日期:2005-02-25 发布日期:2005-02-25

The Preliminary Research on the Morphological and Physiological Responseto Heat Stress of Lilium longiflorum Seedlings

Zhou Sijian;Yi Mingfang;Mu Ding

(¹Department of Ornamental Horticulture and Landscape, China Agricultural University, Beijing 100094, China; ² Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2004-06-24 Revised:2004-11-17 Online:2005-02-25 Published:2005-02-25

摘要/Abstract

摘要： 对离体扩繁的铁炮百合‘Snow Queen’幼苗进行不同时间、不同高温( 35℃/30℃、37℃/32℃、39℃/34℃) 处理, 测定了不同处理对幼苗外观形态和叶片中游离脯氨酸(pro) 、丙二醛(MDA) 、可溶性蛋白质含量和相对电导率。结果表明, 铁炮百合幼苗在37℃/32℃高温处理下, pro含量、MDA含量和相对电导率明显上升, 且3种指标之间具有显著相关性, 可以作为耐热性鉴定指标。37℃/32℃处理下,幼苗外观形态与生理指标变化基本一致, 能反应出幼苗耐热性。35℃/30℃、39℃/34℃处理下各指标间不具显著相关性。

关键词: 铁炮百合, 热胁迫, 游离脯氨酸, 丙二醛, 可溶性蛋白, 相对电导率

Abstract: Seedlings of Lilium longiflorum‘Snow Queen’were treated in chambers with temperatures of 35℃/30℃, 37℃/32℃, 39℃/34℃ for 1, 2, 4, 8, 16 and 32 h, respectively. The changes of the morphology and contents of proline, MDA, soluble protein as well as relative electrical conductivitywere determined. The results showed the contents of proline and MDA, the relative electrical conductivity increased significantly by 37℃/32℃ treatment. Those indexes had a remarkable correlation and could be taken as heat tolerance indexes. The change in morphology of the seedlings under 37℃ treatment was also consistent with that of physiological indexes. The physiological indexes of 35℃ and 39℃ treatment didnpt have significant correlation.

Key words: Lilium longiflorum, Heat stress, Free proline, Malondialdehyde (MDA), Soluble protein, Relative electrical conductivity

中图分类号:

S 682.2

周斯建;义鸣放;穆　鼎. 高温胁迫下铁炮百合幼苗形态及生理反应的初步研究[J]. 园艺学报, 2005, 32(1): 145-147.

Zhou Sijian;Yi Mingfang;Mu Ding. The Preliminary Research on the Morphological and Physiological Responseto Heat Stress of Lilium longiflorum Seedlings[J]. ACTA HORTICULTURAE SINICA, 2005, 32(1): 145-147.

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