关键词: 萝卜, RsCYP86MF基因, cDNA, 克隆, 分析

Abstract: By SMART RACE RT-PCR strategywith gene specific p rimers, the full-length cDNA of a cytochrome P450 gene named RsCYP86M F was cloned from Raphanus sativus L. The cDNA was 1818 bp, it encompassed a open reading frame (ORF) with 1581 bp encoding 526 amino acid residues. the sequences of deduced amino acids encoded by cytochrome P450 genes showed the high identity of 90% between Raphanus sativus L. and Brassica campestris subsp. chinensis, and also highly conserved domains of function. It had a conserved domain of cytochrome P450 cysteine heme-iron ligand signature ( FNAGPRLCIG) , which is typical of cytochrome P450, and the structure domains such as N-glycosylation.

Key words: Raphanus sativus L., RsCYP86M F gene, cDNA, Cloning, Characterization