桃果实有毛/无毛性状的SCAR标记

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1003-1007.

桃果实有毛/无毛性状的SCAR标记

姜立杰;杨英军;张晓明;李文生;张开春

(¹ 北京市农林科学院林业果树研究所, 北京100093; ² 河南科技大学农学院, 洛阳471003)

收稿日期:2005-02-03 修回日期:2005-06-15 出版日期:2005-12-25 发布日期:2005-12-25

SCAR Marker Linked to the Peach/Nectarine in Peach

Jiang Lijie;Yang Yingjun;Zhang Xiaoming;Li Wensheng;Zhang Kaichun

( ¹ Institute of Forestry and Pomology, Beijing Academ y of Agricultural and Forestry Sciences, Beijing 100093, China; ²College of Agriculture, Henan Science-Technology University, Luoyang 471003, China)

Received:2005-02-03 Revised:2005-06-15 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 以桃品种‘京玉’和‘美味’的正反交69株F1 群体为试材, 利用RAPD技术扩增出了与桃果实有毛/无毛性状(G/ g) 连锁的2 258 bp的多态性片段, 经克隆、测序后, 根据获得的序列重新设计了两对引物进行SCAR转化。引物对BFP94 /BFP95在有毛和无毛个体中均扩增出了2 258 bp的片段, RAPD显示的多态性消失。利用引物对BFP96 /BFP98成功将RAPD标记转化成了SCAR标记, 并命名为SCP2022258。该标记仅在果实有毛的个体中出现, 与有毛/无毛性状的连锁距离为718 cM, 且扩增稳定, 为桃果实有毛/无毛性状育种的分子标记辅助选择奠定了基础。

关键词: 桃, 油桃, RAPD, SCAR

Abstract: RAPD technique was used in screen molecular marker linked to peach/nectarine character (G / g) in 69 F₁ hybrids resulting from the intraspecific cross between peach cultivar‘J ingyu’and nectarine cultivar‘Flavortop’. The OPP20 primer resulted in a reproducible bright polymorphic band of 2 258 bp present in the individuals of peach character and peach cultivar‘Jingyu’. The polymorphic band was cloned and sequenced. Four primers were designed based on the sequence to transform into SCAR marker. But primer combination BFP94 /BFP95 amp lified the same-sized band in all individuals resulting in a loss of the original RAPD polymorphism. The RAPD markerwas successfully converted into a SCAR markerwith primer combination BFP96 /BFP98, which was designated SCP2022258. The linkage distance is 718 cM between the SCP2022258 and the peach /nectarine character. The SCAR marker will be useful for marker-assisted selection for peach /nectarine character in peach breeding programs.

Key words: Peach, Nectarine, RAPD, SCAR

中图分类号:

S 662.1

姜立杰;杨英军;张晓明;李文生;张开春. 桃果实有毛/无毛性状的SCAR标记[J]. 园艺学报, 2005, 32(6): 1003-1007.

Jiang Lijie;Yang Yingjun;Zhang Xiaoming;Li Wensheng;Zhang Kaichun. SCAR Marker Linked to the Peach/Nectarine in Peach[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1003-1007.

[1]	翟含含, 翟宇杰, 田义, 张叶, 杨丽, 温陟良, 陈海江. 桃SAUR家族基因分析及PpSAUR5功能鉴定[J]. 园艺学报, 2023, 50(1): 1-14.
[2]	袁馨, 徐云鹤, 张雨培, 单楠, 陈楚英, 万春鹏, 开文斌, 翟夏琬, 陈金印, 甘增宇. 猕猴桃后熟过程中ABA响应结合因子AcAREB1调控AcGH3.1的表达[J]. 园艺学报, 2023, 50(1): 53-64.
[3]	邢柱东, 吕福堂, 郭尚敬, 张演义. 新品种‘聊大红金’桃[J]. 园艺学报, 2023, 50(1): 225-226.
[4]	杨兴旺, 王海波, 王莹莹, 王小龙, 王志强, 刘培培, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农甘爽’[J]. 园艺学报, 2022, 49(S2): 15-16.
[5]	杨兴旺, 王海波, 王莹莹, 张艺灿, 王宝亮, 刘培培, 史祥宾, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农白干’[J]. 园艺学报, 2022, 49(S2): 17-18.
[6]	杨兴旺, 刘凤之, 王海波, 王莹莹, 王志强, 史祥宾, 冀晓昊, 刘万春, 王孝娣. 中熟抗寒桃新品种‘中农寒水蜜’[J]. 园艺学报, 2022, 49(S2): 19-20.
[7]	杨兴旺, 刘凤之, 王海波, 王莹莹, 张艺灿, 李鹏, 王小龙, 刘万春, 王孝娣. 晚熟抗寒桃新品种‘中农秋香’[J]. 园艺学报, 2022, 49(S2): 21-22.
[8]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 极晚熟桃新品种‘中农冬蜜’[J]. 园艺学报, 2022, 49(S2): 23-24.
[9]	王莹莹, 刘立常, 刘志伍, 杨兴旺, 刘万春, 王孝娣, . 小果油桃新品种‘中农珍珠’[J]. 园艺学报, 2022, 49(S2): 25-26.
[10]	吴延军, 刘庆忠, 陈鸿才, 戚行江, 朱东姿, 郑家祥, 曹学敏, 方丹燕. 甜樱桃新品种‘江南锦’[J]. 园艺学报, 2022, 49(S2): 29-30.
[11]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[12]	宋放, 陈奇, 袁炎良, 陈沙, 尹海军, 蒋迎春, . 黄肉猕猴桃新品种‘先沃1号’[J]. 园艺学报, 2022, 49(S2): 47-48.
[13]	齐永杰, 高正辉, 马娜, 王清明, 柯凡君, 陈钱, 徐义流, . 黄肉抗溃疡病猕猴桃新品种‘皖农金果’[J]. 园艺学报, 2022, 49(S2): 49-50.
[14]	张慧琴, 楼国荣, 陆玲鸿, 古咸彬, 宋根华, 谢鸣. 黄肉中华猕猴桃新品种‘金义’[J]. 园艺学报, 2022, 49(S2): 51-52.
[15]	季琳琳, 陈素传, 吴志辉, 常君, 韩文妍, 陶汝鹏. 早花山核桃新品种‘宁国山核桃2号’[J]. 园艺学报, 2022, 49(S2): 53-54.