‘丰香’草莓叶片高效再生体系的建立

园艺学报 ›› 2005, Vol. 32 ›› Issue (1): 101-104.

‘丰香’草莓叶片高效再生体系的建立

秦永华;张上隆;徐　凯;吴延军;秦巧平

(¹浙江大学园艺系, 农业部园艺植物生长发育与生物技术重点开放实验室, 杭州310029;² 安徽农业大学园艺系, 合肥230036)

收稿日期:2004-03-09 修回日期:2004-05-24 出版日期:2005-02-25 发布日期:2005-02-25

A Highly Eff icient System Establishmen t of Shoot Regeneration from LeafExplants of Strawberry

Qin Yonghua;Zhang Shanglong;Xu Kai;Wu Yanjun;Qin Qiaoping

( ¹Department of Horticulture, Zhejiang University, The Ministry of Agricultural Laboratory of Horticultural Plant Growth, Development and Biotechnology, Hangzhou 310029, China; ²Department of Horticulture, Anhui Agricultural University, Hefei 230036, China)

Received:2004-03-09 Revised:2004-05-24 Online:2005-02-25 Published:2005-02-25

摘要/Abstract

摘要： 以草莓主栽品种丰香( Fragaria ×ananassa Duch ‘Toyonoka’) 叶片为外植体, 研究了影响组织培养的多个因素, 建立了高效再生体系。结果表明: MS + TDZ 1.5 mg·L ^{- 1} + IBA 0.4 mg·L^{- 1}培养基最适于不定芽分化。不同滤光膜(绿膜、红膜、蓝膜、黄膜) 对草莓不定芽的分化具显著效应, 绿膜和红膜对芽的分化有明显促进作用, 不定芽再生率达95%以上, 平均每个外植体再生芽数在25个以上; 而蓝膜和黄膜则不利于芽的分化。不同滤光膜光谱差异主要集中在300～700 nm, 红、绿膜在该波段光强较弱,而黄、蓝膜和荧光灯较强。暗处理4周比1、2、3周更有利于提高叶片的不定芽再生率, 此后转到光下培养, 获得的不定芽再生率可达100%。

关键词: 草莓, 滤光膜, 不定芽再生, 暗处理

Abstract: An efficient and reliable method for shoot regeneration from leaf disks of strawberry (Fragaria ananassa Duch ‘Toyonoka’) was established. The optimum medium for shoot regeneration was MS supplemented with thidiazuron ( TDZ) 1.5 mg·L ^{- 1} and Indole-3-butyric acid ( IBA) 0-4 mg·L^{- 1}. Different color films had great effect on the shoot regeneration of strawberry. The presence of red and green films induced the maximum percentage of shoot regeneration ( > 95% ) and the highest number of buds per explant( > 25). However, the blue and yellow films inhibited the shoot regeneration of strawberry. The difference of films spectrum was focused on 300 - 700 nm. The light intensity of red and green films was weaker than yellow, blue and white ones. Dark treatment could effectively p romote shoot regeneration rate. In contrast, maximum percentage of shoot regeneration (100% ) and the highest number of buds per explant (9.05) were observed with dark treatment of four weeks.

Key words: Strawberry, Color plastic films, System of regeneration, Dark treatment

中图分类号:

s668.4

秦永华;张上隆;徐　凯;吴延军;秦巧平. ‘丰香’草莓叶片高效再生体系的建立[J]. 园艺学报, 2005, 32(1): 101-104.

Qin Yonghua;Zhang Shanglong;Xu Kai;Wu Yanjun;Qin Qiaoping. A Highly Eff icient System Establishmen t of Shoot Regeneration from LeafExplants of Strawberry[J]. ACTA HORTICULTURAE SINICA, 2005, 32(1): 101-104.

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