关键词: 梨, 自花授粉, 异花授粉, 花柱, 自发荧光, 激光共聚焦显微镜

Abstract: The variations of style auto—fluorescence after self-and cn)ss—pollination in serotina Rehd．were studied with laser scanning confocal microscope(LSCM)．The pollination combinations were as follows：‘Housui’(S3S5) × ‘Housui’ and‘Housui’ × ‘Nijiseiki’ (S2S4)． Ihe style auto-fluores—
cence increased after pollination and the most remarkable increases occurred in the stigma an d the a瑚0—400 pm down(total length of style is about 3700／nn)．After self-pollination．with a rapid decline of auto-fluorescence from stigma to 950 tan down，there was almost no variation from 950 tan to the end of style． But a visible climbing of auto-fluorescence can be detected from 950 tan to the end of style after cro~s—po llination． Results also showed
that there were diferences of style auto—fluorescence distribution between self-and cIDss—po lination treatments．AⅡtreatments proved that in the upper half of the style the auto—fluorescence in the parietal part was stronger than that in the middle part and so did that in the lower part after self-polina tion． But after cn)ss—polination the au to-fluo rescence was relatively evenly distributed in the lower half of the style．All above indicated that，with LSCM ，visible changes in the style after various pollination treatments Can be detected and thus another method to study the self-incompatibility in vivo or senti in vivo Can be established．

Key words: serotiruz Re